| Field | Specification |
|---|---|
| Applications | |
| Assay type | |
| Sensitivity | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This accessory kit supplies the plates, sealers, buffers, TMB substrate and stop solution used to run EZ-Set™ antibody-pair ELISAs. It contains no antibodies and no standard; use it together with an EZ-Set™ antibody-pair kit.
Kit Components
| 96-well removable strip microplate | 5 |
|---|---|
| Adhesive plate sealers | 20 |
| Reagent Diluent (10X) | 20 mL/vial ×2 |
| Stop Solution | 10 mL/vial×5 |
| Color Developing Reagent (TMB) | 10 mL/vial×5 |
| PBS(20 X) | 25 mL/vial ×2 |
| PBS-T (20 X) | 25 mL/vial ×2 |
Kit components are not sold separately.
Performance Data
| Format | Reagents for five 96-well plates |
|---|
Storage & Handling
Store at 4 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on wet ice.
Safety
Kit components
| PART | PART # | DESCRIPTION | QUANTITY |
| Microplates | AR1100 | 96-well removable strip microplate | 5 |
| Plate Sealers | EK-SEA | Adhesive strips | 20 |
| Reagent Diluent (10X) | AR1106 | 10% BSA in PBS | 20 mL/vial ×2 |
| Stop Solution | AR1105 | 2N H2SO4 | 10 mL/vial×5 |
| Color Developing Reagent | AR1104 | TMB | 10 mL/vial×5 |
| PBS (20 X) | AR2100 | 25 mL/vial ×2 | |
| PBS-T (20 X) | AR2101 | 2% Tween® 20 in PBS | 25 mL/vial ×2 |
Materials required but not provided
- Microplate reader in standard size.
- Automated plate washer.
- Incubator.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- 96 well microplate (Cat# AR1100)
- Plate Sealers.
- Capture Antibody Diluent: PBS.
- Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- Stop Solution: 2 N H2SO4 (Cat# AR1105)
- Wash Buffer (PBS and PBS-T). Recipes:
- PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
- PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
- *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
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