| Field | Specification |
|---|---|
| Target | |
| Alternative names | F8; F8C; Coagulation factor VIII; Antihemophilic factor; AHF; Procoagulant component |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant fragment of Factor VIII expressed in E. Coli. |
| Conjugate | |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation| factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.
Product Overview
This monoclonal antibody, raised in Mouse, recognizes F8. It is validated for Western Blot, ELISA.
Validated Applications
| Applications | Western Blot, ELISA |
|---|
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Purified recombinant fragment of Factor VIII expressed in E. Coli.
Specifications
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Immunogen | Purified recombinant fragment of Factor VIII expressed in E. Coli. |
| Purification | Affinity purification |
| Concentration | 1 mg/ml |
| Formulation | Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol. |
| UniProt Accession | Human: P00451; Mouse: Q06194 |
| Gene ID | Human: 2157 |
| Storage | -20°C for 1 year |
| Lead Time | Within one week |
Reactivity Notes
Validated reactivity: Human. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for F8 research from Bioassay Technology Laboratory, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
