| Field | Specification |
|---|---|
| Target | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human FAK around the non-phosphorylation site of Tyr407. |
| Conjugate | |
| Purification | |
| Concentration | |
| Storage | |
| Storage buffer | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes FAK. It is validated for Western Blot, Immunohistochemistry.
Validated Applications
| Applications | Western Blot, Immunohistochemistry |
|---|---|
| WB | 1:500-1:2000 |
| IHC | 1:100-1:300 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Synthesized peptide derived from human FAK around the non-phosphorylation site of Tyr407.
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthesized peptide derived from human FAK around the non-phosphorylation site of Tyr407. |
| Purification | Affinity Purification |
| Concentration | 1 mg/mL |
| Storage Buffer | PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH 7.4 |
| Calculated MW | 119kDa |
| Observed MW | 119kDa |
| UniProt # | Q05397 |
| Lead Time | 2-3 business days |
| Validity | 12 months |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice packs |
| Target Synonyms | FADK 1;FADK;FAK related non kinase polypeptide;FAK1;FAK1;Focal adhesion kinase 1;Focal adhesion Kinase;Focal adhesion kinase isoform FAK Del33;Focal adhesion kinase related nonkinase;FRNK;p125FAK;pp125FAK;PPP1R71;Protein phosphatase 1 regulatory subunit 71;Protein tyrosine kinase 2;Protein-tyrosine kinase 2;Ptk2;PTK2 protein tyrosine kinase 2 |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for FAK research from Elabscience Biotechnology Co Ltd, or contact us for alternative formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
