| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Assay Type | |
| Detection Range | |
| Product Type | |
| Sample Type(s) | cell culture supernatant, serum, plasma |
| Sensitivity | |
| Storage | |
| Target |
Assay Principle
The Fast Human AMH ELISA is a solid phase ELISA designed to measure human AMH levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 1 hour, with no need for 5-6 hours (Fig. 1). The detection range is from 31 to 2000 pg/mL. The levels of human AMH samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human AMH protein.
Typical Data This standard curve (R 2 =1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Sensitivity The minimum detectable dose (MOD) of human AMH is typically 5 pg/ml.
Fast Anti-Mullerian Hormone/AMH Competitive ELISA Kit is a Competitive ELISA for the quantitative determination of Anti-Mullerian Hormone/AMH. In this competitive ELISA, sample analyte competes with a labeled competitor for a limited number of antibody binding sites, so signal is inversely proportional to analyte concentration.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | Anti-Mullerian Hormone/AMH |
|---|---|
| Sample Type | cell culture supernatant, serum, plasma |
| Assay Type | Competitive |
| Test Principle | Competitive ELISA |
| Sensitivity | 5 pg/mL |
| Detection Range | 31–2000 pg/mL |
| Assay Time | 1 hour |
| Standard | Recombinant Anti-Mullerian Hormone/AMH |
| Specificity | This assay recognizes natural and recombinant human AMH. |
| Storage | Store the unopened kit at 2-8°C. | Components: Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8°C.; Aliquot and store at -20°C for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.; May be stored for up to 3 months at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for cell culture supernatant, serum, plasma. Other matrices may require additional in-lab validation.
This assay has a sensitivity of 5 pg/mL and a detection range of 31–2000 pg/mL. Samples reading outside the range should be diluted or concentrated.
The total assay time is approximately 1 hour from sample addition to plate reading.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this competitive ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
