| Field | Specification |
|---|---|
| Mfr No | |
| Assay Type | |
| Detection Range | |
| Product Type | |
| Sample Type(s) | cell culture supernatant, serum, plasma |
| Sensitivity | |
| Species | |
| Storage | |
| Target |
Interleukin 8 (IL-8, also as CXCL8) is a chemokine with a defining CXC amino acid motif, and has tumorigenic and proangiogenic properties. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL1, TNF, LPS, etc). This chemokine has crucial roles in various pathological conditions such as chronic inflammation and cancer. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer.
Assay Principle
The Canine (Dog) IL-8 Fast ELISA is a solid phase ELISA designed to measure canine/dog IL-8 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1-2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of canine IL-8 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural canine IL-8 protein.
Fast Canine IL-8/CXCL8 ELISA Kit is a Solid-phase sandwich ELISA for the quantitative determination of IL-8/CXCL8. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | IL-8/CXCL8 |
|---|---|
| Species | Canine |
| Sample Type | cell culture supernatant, serum, plasma |
| Assay Type | Sandwich |
| Test Principle | Solid-phase sandwich ELISA |
| Sensitivity | 7.8 pg/mL |
| Detection Range | 8–2000 pg/mL |
| Precision | Intra-assay CV 3.8%; Inter-assay CV 10% |
| Standard | Recombinant Canine IL-8/CXCL8 |
| Specificity | This assay recognizes natural and recombinant canine IL-8. |
| Storage | Store the unopened kit at 2-8°C. | Components: The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8°C.; Aliquot and store at -20°C for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.; May be stored for up to 3 months at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for cell culture supernatant, serum, plasma. Other matrices may require additional in-lab validation.
This assay has a sensitivity of 7.8 pg/mL and a detection range of 8–2000 pg/mL. Samples reading outside the range should be diluted or concentrated.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
