| Field | Specification |
|---|---|
| Target | |
| Species | |
| Applications | |
| Assay type | |
| Sample type(s) | serum, plasma |
| Sensitivity | |
| Detection range | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
Tribioscience’s Fast Human Phospho Tau Thr217 (p-Tau217) ELISA is designed to quantitatively detect human p-Tau217 levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The Hands-on time can be within 2 hours, no need for 4-5 hours (Fig. 1). The detection range is from 0.3 to 243 ng/mL. The levels of human p-Tau217 samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human p-Tau217 protein.
Typical Data This standard curve (R 2 =1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity The minimum detectable dose (MOD) of human is typically 200 pg/ml. The Intra-assay CV and the Inter-assay CV are ˂10%.
Fast Human Phospho Tau Thr217 (pT217) ELISA Kit is a Solid-phase sandwich ELISA for the quantitative determination of Phospho Tau Thr217 (pT217). The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | Phospho Tau Thr217 (pT217) |
|---|---|
| Species | Human |
| Sample Type | serum, plasma |
| Assay Type | Sandwich |
| Test Principle | Solid-phase sandwich ELISA |
| Sensitivity | 200 pg/mL |
| Detection Range | 0.3–243 ng/mL |
| Precision | Intra-assay CV 10% |
| Standard | Recombinant Human Phospho Tau Thr217 (pT217) |
| Specificity | This assay recognizes natural and recombinant human pTau-217. |
| Storage | Store the unopened kit at 2-8°C. | Components: Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8°C.; Aliquot and store at -20°C for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.; May be stored for up to 3 months at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
Gin, A. N. (2024). Early Diagnosis of Alzheimer’s Disease and Studying Binding to Opioid Receptors using Whispering Gallery Mode Microtoroid Biosensors. The University of Arizona. https://repository.arizona.edu/handle/10150/672468
