| Field | Specification |
|---|---|
| Mfr No | |
| Assay Type | |
| Product Type | |
| Sensitivity | |
| Species | |
| Storage | |
| Target |
Testosterone is an androgenic steroid hormone, the primary male sex hormone, and an anabolic steroid. It is secreted mainly by the testes in males and the ovaries in females, with smaller amounts also produced by the adrenal glands. Testosterone plays several vital roles, including driving the development of male reproductive organs, supporting sperm production, influencing muscle mass and strength, and maintaining bone density.
Assay Principle
Typical Data This standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity The analytical sensitivity of the assay is 0.24 ng/mL Human Testosterone.
Cross reactivity No obvious cross reaction with other analogues.
Fast Human Testosterone ELISA Kit is a Competitive ELISA for the quantitative determination of Testosterone. In this competitive ELISA, sample analyte competes with a labeled competitor for a limited number of antibody binding sites, so signal is inversely proportional to analyte concentration.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | Testosterone |
|---|---|
| Species | Human |
| Assay Type | Competitive |
| Test Principle | Competitive ELISA |
| Sensitivity | 0.24 ng/mL |
| Storage | Store the unopened kit at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This assay has a sensitivity of 0.24 ng/mL. Samples reading outside the range should be diluted or concentrated.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this competitive ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
