| Field | Specification |
|---|---|
| Mfr No | |
| Detection Range | |
| Product Type | |
| Sample Type(s) | serum, plasma |
| Sensitivity | |
| Species | |
| Storage | |
| Target |
Assay Principle
Tribioscience’s Fast Human H2AX ELISA is designed to quantitatively detect human H2AX levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The Hands-on time can be within 2 hours, and not need 4-5 hours (Fig. 1). The detection range is from 0.3 to 20 ng/mL. The levels of human H2AX samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human H2AX protein.
Typical Data This standard curve (R 2 =1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity The minimum detectable dose (MOD) of humans is typically 300 pg/ml.
Fast Human Total-H2AX ELISA Kit is a Solid-phase ELISA for the quantitative determination of Total-H2AX. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | Total-H2AX |
|---|---|
| Species | Human |
| Sample Type | serum, plasma |
| Test Principle | Solid-phase ELISA |
| Sensitivity | 300 pg/mL |
| Detection Range | 0.3–20 ng/mL |
| Precision | Intra-assay CV 10% |
| Standard | Recombinant Human Total-H2AX |
| Specificity | This assay recognizes natural and recombinant human H2AX. |
| Storage | Store the unopened kit at 2-8°C. | Components: Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8°C.; Aliquot and store at -20°C for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.; May be stored for up to 3 months at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for serum, plasma. Other matrices may require additional in-lab validation.
This assay has a sensitivity of 300 pg/mL and a detection range of 0.3–20 ng/mL. Samples reading outside the range should be diluted or concentrated.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
