| Field | Specification |
|---|---|
| Applications | |
| Storage | |
| Shelf life | |
| Catalog no. (Mfr.) | |
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Product Overview
This kit provides a simple, rapid and efficient method for extracting DNA from fresh or frozen tissues. After tissue lysis, the DNA binds to the surface of silica-coated magnetic beads. After rinsing, the high-purity DNA is eluted in buffer TB or deionized water. The purified DNA can be directly used in PCR, Real-time PCR, SNP, STR, next-generation sequencing, pharmacogenomics research and so on. Besides being able to rapidly extract DNA from animal tissues, this kit is also applicable to the extraction of DNA from samples such as blood, cells and bacteria.
The protocol processes 25–30 mg of tissue, with less than 10 mg used for spleen, or 200 µL of blood. Tissue is lysed with Buffer ATL and Proteinase K at 56°C for 30 min, treated with RNase A for 10 min, then bound with Buffer AL, isopropanol and Mag beads PN. The beads are washed with Buffer MB, Buffer DW and twice with 75% ethanol at 1200 rpm for 3 min each, dried for 3–5 min and eluted with 80–100 µL of Buffer TB at 56°C for 10 min. The A260/A280 ratio of pure DNA is 1.7–1.9.
Specifications
| Format | Silica-coated magnetic beads (Mag beads PN) |
|---|---|
| Sample types | Fresh or frozen tissue, blood, cells and bacteria |
| Sample input (tissue) | 25–30 mg; less than 10 mg for spleen |
| Sample input (blood) | 200 µL |
| Purity (A260/A280) | 1.7–1.9 for pure DNA |
| Elution volume | 80–100 µL Buffer TB at 56°C for 10 min |
| Enzymes supplied | Proteinase K (20 mg/mL), RNase A (100 mg/mL) |
Kit Components
| Component | 96 T |
|---|---|
| Buffer ATL | 25 mL |
| Buffer AL | 36 mL |
| Buffer MB | 70 mL |
| Buffer DW | 70 mL |
| Buffer TB | 15 mL |
| Proteinase K (20 mg/mL) | 2 × 1.25 mL |
| RNase A (100 mg/mL) | 0.6 mL |
| Mag beads PN | 4 × 1.25 mL |
Applications
- DNA extraction from fresh or frozen animal tissue
- DNA extraction from blood, cells and bacteria
- PCR and Real-time PCR
- SNP and STR analysis
- Next-generation sequencing and pharmacogenomics research
Storage & Handling
All components can be stably stored in a dry environment at room temperature (15–30°C), with a shelf life of 1 year.
Usage Notes
- Mag beads PN must not be frozen or subjected to high-speed centrifugation.
- Redissolve buffers if crystals form before use.
- Gram-positive bacteria require lysozyme pretreatment.
- Use less than 10 mg of sample for spleen tissue.
- The 75% ethanol wash is not listed among the kit components and must be prepared separately.
- Isopropanol is required at the binding step and is not supplied.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
