| Field | Specification |
|---|---|
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
PNGase F is an amidohydrolase that can cleave high-mannose, hybrid, and complex oligosaccharides linked by asparagine, specifically removing N-linked glycans. However, the conventional PNGase F enzymatic reaction often takes several hours to release antibody N-glycans. Due to the preferential release of glycans, incomplete deglycosylation might result in biased outcomes, with the obtained glycan distribution failing to represent the true composition of therapeutic antibodies. Therefore, rapidly obtaining an accurate N-glycan profile is crucial for glycosylation monitoring during the production of antibodies and antibody-fusion proteins used in biotherapeutics.
Yeasen Fast PNGase F（Glycerol-free）is an optimized recombinant reagent capable of thoroughly and rapidly deglycosylating antibodies, immunoglobulins, fusion proteins, and other glycoproteins within minutes. This enzyme can swiftly and non-selectively remove all N-glycans, enabling direct downstream chromatography or mass spectrometry analysis. Yeasen Fast PNGase F（Glycerol-free） simplifies the experimental procedure while reducing experimental time without compromising sensitivity and reproducibility.
Feature
Fast—Complete and rapid deglycosylation in a few minutes.
High purity—No protease, glycosidase contamination, purity ≥95%.
Non-selective—Quickly and without preference remove all N-glycans.
Good compatibility—Directly used for downstream chromatography or MS spectrometry analysis.
Application
Glycan Sequencing
Glycoprotein Analysis
Recombinant Glycoprotein Expression
Specification
|
English synonym |
Fast PNGase F（Glycerol-free） |
|
Source |
Yeast recombinant expression |
|
Storage buffer |
20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA |
Components
|
Components No. |
Name |
20406ES20 |
20406ES50 |
|
20406-A |
Fast PNGase F |
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in Protein Research applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
One unit (U) is defined as the amount of enzyme that catalyzes the release of 1 µmol of product per minute at 37°C from a defined glycoprotein or oligosaccharide substrate under standard assay conditions.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
PNGase F cleaves between the innermost GlcNAc and asparagine of all N-linked glycans regardless of glycan complexity. Endo H cleaves only high-mannose and hybrid-type N-glycans at the chitobiose core, enabling differentiation of ER-retained from Golgi-processed glycoproteins. Endo S is an IgG-specific endoglycosidase that cleaves N-glycans from the Fc region of IgG antibodies, useful in antibody engineering and glycan remodeling workflows.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
- 1. Wang N, Ji W, Jiao H, et al. A MERS-CoV-like mink coronavirus uses ACE2 as an entry receptor. Nature. 2025;642(8068):739-746. doi:10.1038/s41586-025-09007-w(IF:48.5)
Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.
🔬 What's on offer right now:
10% Off Pre-Designed siRNA Sets — use code SIRNA10
20% Off Transmembrane Proteins — use code TM20
$50 Off All ELISA Kits — auto-applied at checkout (code ELISA50)
50% Off Lab Consumables + Free Shipping on orders $500+
15% Off Proteins from Trusted Suppliers — use code PROTEIN15
$99 Pipette Filler Promotion Package (reg. $236)
Save 10% on your first order of any R&D grade DENARASE® with code DENARASE10 at checkout.
