| Field | Specification |
|---|---|
| Mfr No | |
| Assay Type | |
| Detection Range | |
| Product Type | |
| Sample Type(s) | cell culture supernatant, serum, plasma |
| Species | |
| Storage | |
| Target |
Assay Principle
The Ultra-sensitive Human TGF-β1 ELISA is a solid phase ELISA to measure the low concentration of human TGF-β1 at 0.5pg/mL level in cell culture supernatants, serum, and plasma, which is not possible to be detected with the regular ELISA. The main feature of this kit is the use of a novel approach to replace traditional methods. The kit is designed to ensure short steps, a short incubation time, and only one-step washing out. The measurement can be finished within 2 hours instead of the traditional 4-5 hours. The detection range is between 0.5-2048 pg/mL. The levels of human TGF-β1 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TGF-β1 protein.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Related Products Human IL-1ß ELISA (TBS3219)
Fast Ultra-Sensitive Human TGF-β1 ELISA Kit is a Solid-phase sandwich ELISA for the quantitative determination of Ultra-Sensitive TGF-β1. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | Ultra-Sensitive TGF-β1 |
|---|---|
| Species | Human |
| Sample Type | cell culture supernatant, serum, plasma |
| Assay Type | Sandwich |
| Test Principle | Solid-phase sandwich ELISA |
| Detection Range | 0.5–2048 pg/mL |
| Standard | Recombinant Human Ultra-Sensitive TGF-β1 |
| Storage | Store the unopened kit at 2-8°C. | Components: The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8°C.; Aliquot and store at -20°C for up to 6 months in a manual defrost freezer. Avoid repeated freeze-thaw cycles.; May be stored for up to 3 months at 2-8°C |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for cell culture supernatant, serum, plasma. Other matrices may require additional in-lab validation.
This assay has a detection range of 0.5–2048 pg/mL. Samples reading outside the range should be diluted or concentrated.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
