| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CD 16 antibody; CD 16a antibody; CD16 antibody; CD16a antibody; CD16a antigen antibody; CD16B antibody; CD16b antigen antibody; Fc fragment of IgG antibody; Fc fragment of IgG low affinity IIIa receptor (CD16) antibody; Fc fragment of IgG low affinity IIIa receptor antibody; Fc fragment of IgG receptor IIIa antibody; Fc fragment of IgG; low affinity III; receptor (CD16) antibody; Fc fragment of IgG; low affinity III; receptor for (CD16) antibody; Fc fragment of IgG; low affinity IIIa; receptor (CD16) antibody; Fc fragment of IgG; low affinity IIIa; receptor (CD16a) antibody; Fc fragment of IgG; low affinity IIIa; receptor for antibody; Fc fragment of IgG; low affinity IIIb; receptor (CD16b) antibody; Fc fragment of IgG; low affinity IIIb; receptor for (CD16) antibody; Fc gamma R3 antibody; Fc gamma receptor III 2 (CD 16) antibody; Fc gamma receptor III A antibody; Fc gamma receptor IIIA antibody; Fc gamma receptor IIIb (CD 16) antibody; Fc gamma RIII alpha antibody; Fc gamma RIII antibody; Fc gamma RIII beta antibody; Fc gamma RIIIa antibody; Fc gamma RIIIb antibody; Fc of IgG antibody; Fc-gamma receptor III2 (CD 16) antibody; Fc-gamma receptor III2 (CD16) antibody; Fc-gamma receptor IIIb (CD16) antibody; Fc-gamma RIII antibody; Fc-gamma RIII-alpha antibody; Fc-gamma RIIIa antibody; FCG 3 antibody; FCG3 antibody; FCG3A_HUMAN antibody; FCgammaRIIIA antibody; FCGR 3 antibody; FCGR 3A antibody; FCGR3 antibody; FCGR3A antibody; FCGR3A protein antibody; FCGRIII antibody; FCGRIII-2 antibody; FcR 10 antibody; FcR-10 antibody; FcR10 antibody; FcRIII antibody; FcRIIIa antibody; IGFR 3 antibody; IGFR3 antibody; IgG Fc receptor III 1 antibody; IgG Fc receptor III 2 antibody; IgG Fc receptor III-2 antibody; IMD20 antibody; immunoglobulin G Fc receptor III antibody; immunoglobulin G Fc receptor III-2 antibody; Low affinity IIIa receptor antibody; Low affinity immunoglobulin gamma Fc region receptor III A antibody; Low affinity immunoglobulin gamma Fc region receptor III-A antibody; Low affinity immunoglobulin gamma Fc region receptor IIIB antibody; neutrophil-specific antigen NA antibody |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human FCGR3A protein |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a recombinant monoclonal antibody expressed in HEK293F cells. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a monoclonal anti-FCGR3A antibody raised in Human, with confirmed utility in ELISA. It is designed to detect FCGR3A protein in Cynomolgus and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: Recombinant Human FCGR3A protein — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Human): Requires anti-human-IgG secondary reagents for indirect detection.
- Monoclonal format: Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.
- Isotype (IgG1): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Affinity chromatography): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
FCGR3A (also referred to as CD 16 antibody, CD 16a antibody, CD16 antibody, CD16a antibody, CD16a antigen antibody, CD16B antibody, CD16b antigen antibody, Fc fragment of IgG antibody, Fc fragment of IgG low affinity IIIa receptor (CD16) antibody, Fc fragment of IgG low affinity IIIa receptor antibody, Fc fragment of IgG receptor IIIa antibody, Fc fragment of IgG, low affinity III, receptor (CD16) antibody, Fc fragment of IgG, low affinity III, receptor for (CD16) antibody, Fc fragment of IgG, low affinity IIIa, receptor (CD16) antibody, Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody, Fc fragment of IgG, low affinity IIIa, receptor for antibody, Fc fragment of IgG, low affinity IIIb, receptor (CD16b) antibody, Fc fragment of IgG, low affinity IIIb, receptor for (CD16) antibody, Fc gamma R3 antibody, Fc gamma receptor III 2 (CD 16) antibody, Fc gamma receptor III A antibody, Fc gamma receptor IIIA antibody, Fc gamma receptor IIIb (CD 16) antibody, Fc gamma RIII alpha antibody, Fc gamma RIII antibody, Fc gamma RIII beta antibody, Fc gamma RIIIa antibody, Fc gamma RIIIb antibody, Fc of IgG antibody, Fc-gamma receptor III2 (CD 16) antibody, Fc-gamma receptor III2 (CD16) antibody, Fc-gamma receptor IIIb (CD16) antibody, Fc-gamma RIII antibody, Fc-gamma RIII-alpha antibody, Fc-gamma RIIIa antibody, FCG 3 antibody, FCG3 antibody, FCG3A_HUMAN antibody, FCgammaRIIIA antibody, FCGR 3 antibody, FCGR 3A antibody, FCGR3 antibody, FCGR3A antibody, FCGR3A protein antibody, FCGRIII antibody, FCGRIII-2 antibody, FcR 10 antibody, FcR-10 antibody, FcR10 antibody, FcRIII antibody, FcRIIIa antibody, IGFR 3 antibody, IGFR3 antibody, IgG Fc receptor III 1 antibody, IgG Fc receptor III 2 antibody, IgG Fc receptor III-2 antibody, IMD20 antibody, immunoglobulin G Fc receptor III antibody, immunoglobulin G Fc receptor III-2 antibody, Low affinity IIIa receptor antibody, Low affinity immunoglobulin gamma Fc region receptor III A antibody, Low affinity immunoglobulin gamma Fc region receptor III-A antibody, Low affinity immunoglobulin gamma Fc region receptor IIIB antibody, neutrophil-specific antigen NA antibody) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of FCGR3A biology in others.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG1 from Human) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Confirm the clone does not cross-react with related family members under your assay conditions.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Cynomolgus. Extrapolation to untested species requires empirical validation.
This product is a recombinant monoclonal antibody expressed in HEK293F cells.
FCGR3A is a protein target in Cynomolgus biology. This monoclonal antibody raised in Human is designed to detect FCGR3A in ELISA applications, with IgG1 isotype.
Reported reactive against Cynomolgus. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG1 antibody requires a compatible secondary. Select anti-human IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG1.
(1) Isotype control — IgG1 from Human at matching concentration; (2) Positive control — known FCGR3A-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
