| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | FEN1; At5g26680; F21E10.3Flap endonuclease 1; FEN-1; EC 3.1.-.-; Flap structure-specific endonuclease 1 |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Arabidopsis thaliana Flap endonuclease 1 protein (85-383AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-FEN1 antibody raised in Rabbit, conjugated with Biotin, with confirmed utility in ELISA. It is designed to detect FEN1 protein in Arabidopsis thaliana and supports researchers working in epigenetics and nuclear signaling contexts.
Key elements and design rationale
- Immunogen: Recombinant Arabidopsis thaliana Flap endonuclease 1 protein (85-383AA) — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
- Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Protein G purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
- Biotin conjugate: Amplified detection via streptavidin/avidin reporters. Block endogenous biotin in biotin-rich tissue samples.
Biological background
FEN1 (also referred to as FEN1, At5g26680, F21E10.3Flap endonuclease 1, FEN-1, EC 3.1.-.-, Flap structure-specific endonuclease 1) is a protein target studied in Arabidopsis thaliana (Mus musculus (Mouse)-ear cress) systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of FEN1 biology in epigenetics and nuclear signaling.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Arabidopsis thaliana. Extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
FEN1 is a protein target in Arabidopsis thaliana biology. This polyclonal antibody raised in Rabbit is designed to detect FEN1 in ELISA applications, with IgG isotype.
Reported reactive against Arabidopsis thaliana. Immunogen derived from Arabidopsis thaliana (Mus musculus (Mouse)-ear cress). Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
Biotin enables streptavidin/avidin-amplified detection. Block endogenous biotin in biotin-rich tissues.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — known FEN1-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
