| Field | Specification |
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Product Overview
This product is suitable for separating and purifying total DNA (including genomic DNA, mitochondrial DNA and any viral DNA present) from 3–8 tissue sections of 10 µm, with a total area of less than 250 mm². The DNA is adsorbed to the purification column, and the degraded protein and PCR inhibitors are removed by the specific washing solution, and the final DNA product is eluted with Buffer TE, so it can be used in various molecular biology experiments such as PCR, sequencing, and enzyme digestion.
Sections are deparaffinised with xylene using a 10 s vortex, washed with ethanol and air-dried, then digested with Proteinase K at 56°C for 1 h and heated at 90°C for 1 h. After addition of the binding buffers the lysate is bound to Adsorption Column G at 12,000 rpm for 30 s, washed with Buffer RP and Buffer WB, dried at 14,000 rpm for 1 min and eluted with 60–100 µL of Buffer TE at 56°C. The expected purity is OD260/OD280 1.7–1.9.
Specifications
| Format | Spin column (Adsorption Column G) |
|---|---|
| Sample type | Formalin-fixed paraffin-embedded animal tissue sections |
| Sample input | 3–8 sections of 10 µm, area less than 250 mm² |
| Nucleic acids recovered | Genomic DNA, mitochondrial DNA and possible viral DNA |
| Purity (OD260/OD280) | 1.7–1.9 |
| Elution volume | 60–100 µL Buffer TE at 56°C |
Kit Components
| Component | 50 T |
|---|---|
| Proteinase K | 1.2 mL |
| Buffer GA1 | 15 mL |
| Buffer GA2 | 12 mL |
| Buffer RP | 28 mL |
| Buffer WB | 32 mL |
| Buffer TE | 12 mL |
| Adsorption Column G | 50 set |
Applications
- Total DNA extraction from FFPE tissue sections
- Recovery of genomic, mitochondrial and possible viral DNA
- PCR from archived tissue
- Sequencing and enzyme digestion
Storage & Handling
Proteinase K is stored at -20°C for 2 years. The other components are stored at room temperature for 1 year. Storage at 2–8°C extends validity beyond 2 years.
Usage Notes
- Discard the first 2–3 tissue slices before collecting sections for extraction.
- Avoid contact between the solutions and the column nozzle.
- Do not omit the drying step, as residues compromise PCR.
- For low yield, extend the digestion time and increase the sample volume.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
