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Hieff NGS™ FFPE DNA Repair Reagent is designed to repair the spectrum of chemical damage in Formalin-Fixed Paraffin-Embedded (FFPE) DNA, including deamination, oxidation, and strand breaks. Compatible with Hieff NGS™ Library Preparation Kits (Cat# 12197 / Cat# 12927) and A-tailing module (Cat# 12608), supporting repair of 5 ng to 1 µg of fragmented FFPE DNA. Also compatible with fragmentation enzyme-based library preparation workflows.
Features
- High Repair Efficiency: optimized enzyme system delivers superior FFPE DNA damage repair
- Streamlined Workflow: single-buffer system compatible with end-repair and dA-tailing — no buffer exchange required
- High Compatibility: directly compatible with Hieff NGS™ library preparation kits
- Broad Input Range: 5 ng to 1 µg fragmented FFPE DNA
Applications
- Repair of low-quality damaged FFPE DNA for NGS library preparation
- Whole-genome, targeted panel, and exome sequencing from FFPE samples
- Tumor genomics, biomarker discovery, and archival tissue genomics
FFPE fixation introduces multiple types of DNA damage including cytosine deamination (C→U mutations), oxidative damage (8-oxoguanine), depurination, cross-linking, and strand breaks. This reagent contains an optimized enzyme cocktail targeting the major classes of FFPE-induced damage, restoring DNA to a state compatible with efficient library preparation and accurate sequencing.
5 ng to 1 µg of fragmented FFPE DNA. Lower inputs are supported but may reduce library yield. Input DNA should be quantified by a dsDNA-specific method (e.g., Qubit fluorometer) rather than UV absorbance, as FFPE DNA preparations often contain absorbance-interfering contaminants.
This reagent is validated and specifically optimized for use with Yeasen Hieff NGS™ Library Preparation Kits (Cat# 12197, Cat# 12927) and the A-tailing module (Cat# 12608), as well as fragmentation enzyme-based library preparation workflows. Compatibility with third-party library preparation kits should be independently validated.
The single-buffer system is compatible with both FFPE DNA repair and the subsequent end-repair/dA-tailing module without requiring buffer exchange between steps. This eliminates a cleanup step, reduces total workflow time, minimizes DNA loss between steps, and simplifies protocol execution.
Supported applications include whole-genome sequencing, targeted gene panel sequencing, whole-exome sequencing, and amplicon sequencing from FFPE-derived libraries. The reagent is designed to maximize library complexity and reduce sequence artifacts introduced by unrepaired damage sites, which would otherwise generate false-positive variant calls.
Yeasen Biotechnology supports custom formulation and scale-up services for reagent mixes and master mixes. Contact BioHippo to discuss project requirements and initiate an inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen reagent mixes are available in GMP grade for IVD assay development. Manufactured in an ISO 13485-certified UCF.ME™ facility with FDA Drug Master File (DMF) support.
- GMP release testing and CoA documentation
- Scalable from milliliter to liter-scale quantities
- Consistent lot-to-lot performance specifications
▶ Glycerol-Free & Lyophilization-Ready Formats
Glycerol-free and lyophilization-ready formats are available for reagents intended for cartridge-based point-of-care devices, liquid handling automation, or ambient-temperature distribution.
- Glycerol-free liquid formulation
- Lyophilization-compatible format with cryoprotectant
- Custom reaction buffer optimization
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting team provides end-to-end support for molecular diagnostic product development using Yeasen reagent mixes as a foundation.
- Master mix selection and performance matching for target assay
- Primer/probe design and assay buffer optimization
- Sensitivity, specificity, and LOD validation
- Instrument compatibility assessment
▶ Custom Formulation
Custom formulation services are available for researchers requiring modifications to standard reagent mix compositions, including ROX level adjustment, dye substitution, stabilizer optimization, and buffer modification.
- ROX concentration customization (No / Low / High ROX)
- Reference dye and indicator dye options
- Buffer composition and pH optimization
- Scale-up to commercial quantities on confirmation
ⓘ Customization services fulfilled by Yeasen Biotechnology. Contact BioHippo for project scoping and pricing.
