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Product Overview
This kit is suitable for effectively purifying total RNA from formalin-fixed and paraffin-embedded tissues. It is suitable for extracting high-purity total RNA from wax-embedded tissues or sections weighing less than 30 mg. This kit does not require the use of phenol/chloroform extraction and isopropanol precipitation. Multiple samples can be extracted within one hour. This product uses specially optimized lysis buffer and proteinase K to release RNA from formalin-fixed or tissue section samples. No overnight operation is required; after digestion of the sample, incubation at a higher temperature removes the inhibitory effect caused by formalin cross-linking, effectively releasing RNA from tissue sections while avoiding damage to the integrity of RNA; the optimized buffer system enables RNA in the lysis buffer to specifically bind to the silica gel adsorption membrane, while other contaminants can flow through the membrane; it can be effectively removed through the rinsing step, and the eluted RNA can be directly used for RT-PCR, Real-Time PCR and blotting analysis and other experiments.
Paraffin is removed by one of two procedures (Plan A or Plan B in the manual). Buffer GTL and Proteinase K are then added and the sample is incubated at 56°C for 15 minutes followed by 80°C for 15 minutes. After cooling on ice and centrifugation, Buffer GL and ethanol are added and the lysate is bound to the Spin Column DM. An optional DNase I treatment is available at this step, followed by two Buffer RW2 washes, drying and elution in 20–50 µL of RNase-Free Water.
Specifications
| Format | Spin column (Spin Columns DM, silica gel adsorption membrane) |
|---|---|
| Sample type | Formalin-fixed paraffin-embedded tissue or sections |
| Sample input | Less than 30 mg |
| Processing time | Multiple samples within one hour |
| Chemistry | No phenol/chloroform extraction, isopropanol precipitation or overnight step |
| Special treatment | Optional DNase I; 1000 U with 10× Reaction Buffer supplied |
| Elution volume | 20–50 µL RNase-Free Water |
Kit Components
| Component | 50 T |
|---|---|
| DNase I | 1000 U |
| 10× Reaction Buffer | 1000 µL |
| Buffer DS | 30 mL |
| Buffer GTL | 15 mL |
| Buffer GL | 25 mL |
| Proteinase K | 12.5 mg |
| Proteinase K Storage Buffer | 1.25 mL |
| Buffer RW1 | 40 mL |
| Buffer RW2 (concentrate) | 11 mL |
| RNase-Free Water | 10 mL |
| Spin Columns DM with Collection Tubes | 50 |
| RNase-Free Centrifuge Tubes (1.5 mL) | 50 |
Applications
- Total RNA purification from formalin-fixed paraffin-embedded tissue
- RNA extraction from wax-embedded sections under 30 mg
- RT-PCR and Real-Time PCR from archived tissue
- Blotting analysis
Storage & Handling
DNase I and the 10× Reaction Buffer are stored at -20°C; the other components are stored at room temperature (15–30°C). The shelf life is 1 year at room temperature.
Usage Notes
- Use RNase-free materials and disposable masks and gloves.
- Samples should be fixed in 4%–10% formalin for 14–24 hours.
- Avoid long-term room temperature storage of Proteinase K.
- Ethanol is required at the binding step and to prepare Buffer RW2; it is not supplied.
- Check the buffers for crystals before use.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
1,25(OH)₂D₃ inhibits ferroptosis in nucleus pulposus cells via VDR signaling to mitigate lumbar intervertebral disc degeneration. Scientific Reports, 2025. 10.1038/s41598-025-92405-x
