| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Flagellar filament 35 kDa core protein, flaB, 35 kDa antigen, Flagellin class B, flaB2, LA_2019 |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai flaB protein (1-283aa) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-flaB antibody raised in Rabbit, with confirmed utility in ELISA, WB. It is designed to detect flaB protein in Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai flaB protein (1-283aa) — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
- Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Protein G purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
flaB (also referred to as Flagellar filament 35 kDa core protein, flaB, 35 kDa antigen, Flagellin class B, flaB2, LA_2019) is a protein target studied in Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of flaB biology in others.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
- WB: Confirms target molecular weight and distinguishes isoforms under denaturing conditions.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai. Extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
flaB is a protein target in Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai biology. This polyclonal antibody raised in Rabbit is designed to detect flaB in ELISA, WB applications, with IgG isotype.
Reported reactive against Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai. Immunogen derived from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601). Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA, WB. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG antibody requires a compatible secondary. Select anti-rabbit IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — known flaB-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
