Flag-Tag (1C1) Mouse mAb

What it means: Monoclonal antibodies originate from a single hybridoma clone that recognizes one defined epitope on the target antigen — making Monoclonal antibodies uniquely consistent between lots.

Why it matters:

• Monoclonal antibodies offer lot-to-lot reproducibility that is critical for longitudinal studies, quantitative assays, and any experimental design requiring matched performance across timepoints or collaborating labs.
• The defined single-epitope binding profile enables reliable pairing with a non-competing capture antibody for sandwich ELISA, proximity ligation, and flow cytometry multiplex panels.

How to interpret here: A single, well-optimized titration for this Monoclonal antibody transfers reliably across experiments. Confirm whether the target epitope is preserved under your fixation or denaturation conditions before proceeding with IHC or WB applications.

What it means: This antibody was raised in Mouse, producing monoclonal-format IgG produced in mouse hybridomas, offering exceptional lot-to-lot consistency. Anti-mouse secondary antibodies are the most widely catalogued in the industry.

Why it matters:

• The Mouse host species determines which secondary antibody you need for indirect detection — select an anti-Mouse IgG secondary matched to your detection label (HRP, fluorochrome, or biotin).
• In experiments with mouse-origin samples, use anti-mouse secondary antibodies that are pre-adsorbed against mouse IgG, or switch to isotype-specific secondaries to prevent background from endogenous mouse immunoglobulins.

How to interpret here: Confirm availability of an anti-Mouse secondary antibody in your lab before ordering. For multiplex IF, pair this Mouse-raised antibody with antibodies from a different host species to avoid secondary antibody cross-reactivity.

What it means: This antibody has validated reactivity to ALL antigen, confirming it binds the ALL ortholog of the target protein in immunoassay conditions.

Why it matters:

• Reactivity to ALL is confirmed through experimental validation — not merely predicted from sequence homology — ensuring reliable performance in ALL-derived samples such as cell lysates, tissues, or serum.
• Use of this antibody with non-ALL samples requires independent validation; predicted cross-reactivity should always be confirmed empirically.

How to interpret here: Ensure your experimental samples are of ALL origin. If you require reactivity to additional species, contact our team to identify a cross-reactive alternative.

What it means: IgG1 is the immunoglobulin class and subclass of this antibody, defining its constant-region structure and effector functions.

Why it matters:

• Isotype IgG1 determines which secondary antibody constant-region specificities are compatible — isotype-specific anti-IgG1 or anti-IgG2a secondaries enable discrimination of co-stained antibodies from the same host species in multiplex experiments.
• For functional assays, isotype identity guides selection of Fc-receptor binding controls and isotype-matched negative controls required for rigorous experimental design.

How to interpret here: Use a negative control of the same isotype (IgG1) to assess non-specific binding in your assay. If performing multiplex staining with multiple antibodies from the same species, select isotype-specific secondary antibodies to resolve each IgG1 signal independently.

What it means: This antibody has been experimentally validated for 1 application: Western Blot.

Why it matters:

• Western Blot
• Each listed application represents an independently optimized protocol — not a theoretical prediction — giving you confidence that the antibody will perform reliably when used at the recommended conditions for that technique.

How to interpret here: Select the application that matches your assay (Western Blot) and refer to the datasheet for the validated dilution range specific to each technique. Using this antibody outside the listed validated applications requires independent optimization and performance verification.