| Field | Specification |
|---|---|
| Target | |
| Alternative names | DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag |
| Host | |
| Clonality | |
| Isotype | |
| Applications | |
| Immunogen | Synthetic Peptide |
| Conjugate | |
| Concentration | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
Product Overview
This monoclonal antibody, raised in Mouse, recognizes Flag-Tag (HRP Conjugated). It is validated for Western Blot.
Validated Applications
| Applications | Western Blot |
|---|---|
| WB 1 | 5,000-10,000 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Synthetic Peptide
Specifications
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Immunogen | Synthetic Peptide |
| Concentration | 1 mg/mL |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
Reactivity Notes
Validated reactivity: see specifications. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for Flag-Tag (HRP Conjugated) research from ELK Biotechnology, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
