| Field | Specification |
|---|---|
| Target | |
| Alternative names | F-ara-ATP |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C10H15FN5O13P3 |
| SMILES | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
Fludarabine triphosphate, also known as F-ara-ATP, is the active metabolite of Fludarabine and a potent, noncompetitive, specific inhibitor of DNA primase, with an IC50 of 2.3 μM and a Ki of 6.1 μM. It inhibits DNA synthesis by blocking DNA primase and primer RNA formation, and it also inhibits ribonucleotide reductase and DNA polymerase, ultimately leading to cellular apoptosis[1][2]. It has the molecular formula C10H15FN5O13P3 and a molecular weight of 525.17 g/mol.
Physical & Chemical Properties
| CAS Number | 74832-57-8 |
|---|---|
| Molecular Formula | C10H15FN5O13P3 |
| Molecular Weight | 525.17 g/mol |
| SMILES | O[C@@H]([C@@H]1O)[C@](O[C@@H]1CO[P](O[P](O[P](O)(O)=O)(O)=O)(O)=O)([H])N2C3=NC(F)=NC(N)=C3N=C2 |
| Target | DNA Polymerase |
| Signaling Pathway | Cell Cycle/DNA Damage; Metabolic Enzyme/Protease; Apoptosis |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with your institution's chemical hygiene plan.
In Vitro
Fludarabine triphosphate inhibits polydeoxythymidylate primase activity more potently than the DNA polymerase α/δ activities in the supernatants of CCRF-CEM cells[1]. At 10-50 μM, Fludarabine triphosphate inhibits incorporation of ATP into primer RNA and of dTTP into DNA to a similar extent[1].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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NAT10-mediated mRNA N4-acetylcytidine reprograms serine metabolism to drive leukaemogenesis and stemness in acute myeloid leukaemia. Nat Cell Biol 2024 Dec;26(12):2168-2182. PMID: 39506072
