| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping |
Background
Tracking the location, abundance, and behavior of defined cell populations is fundamental to cancer and immunology research. Bioluminescence imaging with luciferase enables sensitive, longitudinal monitoring of cell number in living animals, while fluorescent proteins allow single-cell identification by microscopy and flow cytometry. Combining both modalities in the same cell provides complementary readouts: luciferase for whole-body imaging of tumor growth and metastasis, and fluorescence for ex vivo histology, FACS-based isolation, and analysis of tumor-infiltrating immune cells. Stable, division-resistant labeling is essential for accurate longitudinal preclinical studies of tumor biology and the tumor microenvironment.
Product Description & Applications
The Fluorescence and Luciferase Dual Reporter Lentivirus constitutively co-expresses a luminescent reporter (firefly or secreted Gaussia luciferase) and a fluorescent protein (GFP or RFP) from a single transcript, using self-cleaving peptides for independent, equimolar protein production. Expression is driven by a CMV, PGK, or EF1α promoter, and a selection marker (puromycin or blasticidin) supports stable cell line generation. Stable integration ensures persistent dual-reporter expression, enabling longitudinal bioluminescence imaging of tumor growth and metastasis in vivo and parallel fluorescence-based identification, gating, or FACS sorting of tumor cells for flow cytometry, scRNA-seq, and proteomics. Multiple vector layouts optimize for stable line establishment or primary-cell labeling. Supplied as ultra-purified, high-titer lentiviral particles suitable for in vitro and in vivo use, including transduction of primary and cryopreserved cells.
About This Product
This lentivirus constitutively co-expresses a luciferase reporter and a fluorescent protein (GFP, RFP, Luc) from a strong CMV or EF1α promoter, using a self-cleaving P2A peptide to ensure equimolar, independent production of both reporters. A selection marker (Blasticidin, Puromycin) is co-encoded for stable cell line generation.
The stable lentiviral integration ensures persistent, division-stable reporter expression in the target cell line, enabling longitudinal bioluminescence imaging (IVIS) of tumor burden in mouse models and parallel fluorescence-based readouts for ex vivo analysis. The dual-modality design provides built-in redundancy — luciferase for high-sensitivity whole-body imaging and fluorescence for histology, flow cytometry, and FACS — within a single stably engineered cell line.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
