| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C15H8Cl2F3NS |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
Fluorizoline selectively and directly binds to prohibitin 1 (PHB1) and prohibitin 2 (PHB2) and induces apoptosis. It reduces chronic lymphocytic leukemia (CLL) cell viability through upregulation of NOXA and BIM, and it exerts antitumor action in a p53-independent manner[1]. It is supplied as a white to off-white solid (C15H8Cl2F3NS, MW 362.20) at 99.77% purity.
Physical & Chemical Properties
| CAS Number | 1362243-70-6 |
|---|---|
| Molecular Formula | C15H8Cl2F3NS |
| Molecular Weight | 362.20 g/mol |
| Purity | 99.77% |
| Appearance | Solid |
| Color | White to off-white |
| SMILES | FC1(C(F)(N=C(S1)C2=CC=C(C=C2)Cl)F)C3=CC=C(C=C3)Cl |
| Signaling Pathway | Apoptosis |
| Solubility | In Vitro: DMSO: 50 mg/mL (138.05 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 2 years; -20°C, 1 year. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 50 mg/mL (138.05 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 2 years) or -20°C (up to 1 year); avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline |
|---|---|
| Result | 2.5 mg/mL (6.90 mM); suspension; requires sonication |
| How to prepare | Gives a suspension at 2.5 mg/mL. The suspension is suitable for oral and intraperitoneal dosing. For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 400 μL PEG300; then 50 μL Tween-80; then 450 μL saline to bring the volume to 1 mL. Saline: dissolve 0.9 g sodium chloride in ddH2O and make up to 100 mL. |
Protocol 2
| Composition | 10% DMSO + 90% (20% SBE-β-CD in saline) |
|---|---|
| Result | 2.5 mg/mL (6.90 mM); suspension; requires sonication |
| How to prepare | Gives a suspension at 2.5 mg/mL. The suspension is suitable for oral and intraperitoneal dosing. For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 900 μL 20% SBE-β-CD in saline. 20% SBE-β-CD in saline: dissolve 2 g SBE-β-CD powder in 10 mL saline until clear (4°C, store up to one week). |
Protocol 3
| Composition | 10% DMSO + 90% Corn Oil |
|---|---|
| Result | ≥ 2.5 mg/mL (6.90 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.5 mg/mL (saturation not determined). Use with caution if continuous dosing will exceed two weeks. For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 900 μL corn oil. |
Data provided by the manufacturer.
In Vitro
In primary CLL cells ex vivo, Fluorizoline (1.25-20 μM; 24 hours) induces apoptosis[1]. Fluorizoline (5-10 μM; 48 hours) raises NOXA protein levels[1]. The percentage of viable normal B and T cells is reduced by Fluorizoline (48.6% and 82.8% of viable cells after 24 hours of exposure to 10 μM Fluorizoline, in the normal CD19+ and CD3+ populations, respectively), with mean EC50 values of 10.9 μM for normal B cells and 19.1 μM for normal T cells at 24 hours[1].
Apoptosis Analysis[1]
| Cell Line | Primary CLL cells |
|---|---|
| Concentration | 1.25 to 20 μM |
| Incubation Time | 24 hours |
| Result | Strongly reduced cell viability and induced apoptosis in a dose-dependent manner. |
Western Blot Analysis[1]
| Cell Line | Primary CLL cells |
|---|---|
| Concentration | 5, 10 μM |
| Incubation Time | 48 hours |
| Result | Caused an increase of NOXA protein levels. |
In Vivo
In 6-week-old recipient C57BL/6 Eμ-TCL1 mice (a mouse model of CLL), Fluorizoline (15 mg/kg; ip; three times a week for five weeks) leads to very rapid (3 weeks) leukemic development, reflected by a rise in the percentage and number of CD5+CD19+ CLL cells in the blood. No apoptosis is induced by Fluorizoline in vivo. Disease development in the spleen is not controlled by Fluorizoline, as indicated by enlarged spleens[2].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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