FOLR2/NFκB Reporter Lentivirus

Background

FOLR2 (folate receptor beta) is a GPI-anchored cell-surface receptor that binds folate with high affinity and mediates its cellular uptake. It is expressed predominantly on macrophages, including tumor-associated and inflammation-associated macrophages, making it a marker of distinct myeloid populations. Engagement of FOLR2 and receptor-associated signaling can converge on the NF-κB pathway, a master regulator of inflammatory and immune gene expression. NF-κB transcription factors, normally held inactive by IκB proteins, translocate to the nucleus upon stimulation and bind κB response elements. Because FOLR2-positive macrophages shape inflammatory and tumor microenvironments, FOLR2 is an emerging target for immunology and cancer research.

Product Description & Applications

The FOLR2/NFκB Reporter Lentivirus is a two-component immunotherapy reporter system for studying signaling downstream of folate receptor beta. One lentivirus constitutively expresses the human FOLR2 receptor; the second carries an NFκB-responsive dual reporter that produces secreted Gaussia luciferase together with a fluorescent protein (GFP or RFP). Upon ligand binding, FOLR2 activates the NFκB pathway and drives reporter expression, while blocking receptor engagement suppresses the signal, enabling inhibitor studies. Sequential transduction and antibiotic or fluorescence selection generate a stable reporter cell line. Applications include studying macrophage activation, inflammatory signaling, and FOLR2-targeted therapeutics. Supplied as high-titer, VSV-G pseudotyped third-generation lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.

About This Product

This 2-vial immunotherapy reporter system consists of a Vial 1 Receptor Lentivirus encoding human FOLR2 under a constitutive promoter with antibiotic selection, and a Vial 2 Reporter Lentivirus encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP-P2A-GLuc, GFP, RFP). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.

Secreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.