Forskolin

SKU:BHB21300144
Overview
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Forskolin (CAS 66575-29-9) increases cAMP levels by activating adenylate cyclase1. Supplied as Lyophilized powder (purity >97% (HPLC), solubility DMSO, ethanol. Centrifuge all product preparations before use (10000 x g 5 min)). Commonly used in Molecular & Cellular Biology studies, including enzyme activity assays and pathway modulation studies.
Cas No 66575-29-9
concentration 5-10 μM.
purity >97% (HPLC)
Molecular Formula C22H34O7.
Form Lyophilized powder.
Options selector
Catalog no. Size Quantity
F-500-10MG-1 10 mg
F-500-100MG-1 100 mg
F-500-25MG-1 25 mg
F-500-5MG-1 5 mg
F-500-50MG-1 50 mg
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size (5) - 10 mg, 100 mg, 25 mg, 5 mg, 50 mg; Quantity: 1
  • Lead time: typically ships in 1-2 business days; timing may vary by selected option.
  • Storage: After reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage of solutions: Up to four months at 4°C.
  • Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No F-500
Accession Number P0C1Y9
Activity
  • Activator
Cas No. 66575-29-9
Concentration 5-10 μM.
Form Lyophilized powder.
Product Type
  • Biochemicals
  • Small Molecules
Purity >97% (HPLC)
Reconstitution Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
Shipping Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility DMSO, ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
Source Natural
Storage After reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage of solutions: Up to four months at 4°C.

Product details

  • Chemical name: [3R-(3a,4ab,5b,6b,6aa,10a,10ab,10ba)]-5-(Acetyloxy)-3-e thenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7,10a-pent amethyl-1H-naphtho[2,1-b]pyran-1-one.
  • Also known as: Colforsin, Coleonol
  • CAS number: 66575-29-9
  • Molecular formula: C22H34O7.
  • Purity: >97% (HPLC)
  • Concentration: 5-10 μM.
  • Form: Lyophilized powder.
  • Solubility: DMSO, ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
  • Reconstitution: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
  • Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
  • Source: Natural
  • Activity: Forskolin increases cAMP levels by activating adenylate cyclase1.
  • Alternative names: Colforsin, Coleonol

Scientific background

Forskolin is a cell permeable diterpenoid isolated from Coleus forskohlii. Forskolin has a unique ability to stimulate adenylate cyclase activity and increase cyclic adenosine monophosphate (cAMP)1-10.

Lead time: 1-2 Business Days

Country of origin: Israel/IL

Applications key

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Bioassay tested: Yes

Cited application(s)

Electrophysiology

Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.

  • Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
  • Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
  • Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
  • QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
  • Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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