| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
FOXA1 and FOXA2, also known as HNF-3A and HNF-3B, are forkhead-box pioneer transcription factors that bind compacted chromatin and open it to enable the recruitment of additional transcription factors. They are central regulators of endoderm-derived organ development, directing the formation and function of liver, pancreas, and lung. In adult tissues FOXA1/2 control glucose and lipid metabolism and act as cofactors for nuclear hormone receptors, shaping their genomic binding. The factors also influence cell proliferation, differentiation, migration, invasion, and drug resistance. Altered FOXA1/2 activity is implicated in metabolic disease and in several cancers, where their pioneering function affects lineage identity and tumor behavior, making them important targets in developmental biology research.
Product Description & Applications
The FOXA1/2 Reporter Lentivirus is a transcription factor reporter system that detects transcriptional activity mediated by FOXA1 and FOXA2 in mammalian cells. The construct incorporates tandem repeats of consensus DNA-binding elements from the human protein C promoter, placed upstream of a minimal promoter driving a fluorescent or luminescent reporter, with an optimized upstream enhancer that maximizes signal-to-noise and provides specific readout of FOXA1/2 activity. A constitutive drug selection marker (Blasticidin, Hygromycin, Puromycin, or Zeocin) enables generation of stable polyclonal reporter cell lines suitable for fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, the product is well suited to studying FOXA1/2 transcriptional activity in primary and difficult-to-transfect cells for developmental and cancer research.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FOXA1/2 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
