| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
FOXC1 is a forkhead-box transcription factor that binds specific DNA elements to regulate gene expression during development. It is expressed predominantly in the heart, kidney, cartilage, and sternum, where it controls genes such as Col10a1, PTHrP, Gli1, and Ptch1 in chondrocytes. These targets are synergistically stimulated by co-expressed Gli2 and through Ihh-Gli2 signaling during endochondral ossification, linking FOXC1 to skeletal development. Pathological FOXC1 missense mutations, such as those found in Axenfeld-Rieger syndrome, impair chondrocyte proliferation and maturation and disrupt the Gli2-FOXC1 signaling association. FOXC1 is therefore an important factor in developmental biology and skeletal disease research.
Product Description & Applications
The FOXC1 Reporter Lentivirus is a lentiviral reporter system that detects transcriptional activity mediated by FOXC1 in mammalian cells. The construct contains tandem repeats of FOXC1 DNA-binding elements derived from the mouse Col10a1 promoter, driving expression of a fluorescent (GFP, RFP, BFP, mCherry, EGFP, d2GFP) or luminescent (firefly, Renilla, or Gaussia luciferase) reporter. It provides a sensitive readout of FOXC1-driven transcription and the signaling, including Gli2 and Ihh-Gli2 inputs, that modulates it. A range of antibiotic selection markers (blasticidin, hygromycin, puromycin, zeocin) supports establishment of stable reporter cell lines. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it ensures efficient transduction of difficult-to-transfect cells, including primary and thawed cells.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FOXC1 Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
