| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
FOXC2 is a forkhead box transcription factor essential for developmental patterning and tissue maintenance, including lymphatic vessel formation and the development of the lungs, eyes, kidneys, urinary tract, and cardiovascular system. ETS family transcription factors, such as the endothelial regulator ETV2, control endothelial and mesodermal gene programs. FOXC2 and ETS factors can act together at composite regulatory elements, where their synergistic binding drives target genes involved in vascular and lymphatic development. This cooperative FOXC2-ETS activity is important for endothelial differentiation and has relevance to angiogenesis, lymphangiogenesis, and developmental disorders, making the combined transcriptional output an informative readout in vascular biology research.
Product Description & Applications
The FOXC2/ETS Reporter Lentivirus is a transcription-factor reporter system that measures the combined activity of the forkhead factor FOXC2 and the ETS family member ETV2. The construct uses tandem repeats of FOXC2 binding elements derived from the MEF2 enhancer to detect their synergistic transcriptional activity and the activation of related signaling pathways. A fluorescent or luminescent reporter, chosen from options including GFP, RFP, mCherry, BFP2, and luciferase configurations, is paired with a constitutively expressed selection marker for stable polyclonal cell line generation.
Readout is by microscopy, flow cytometry, or luminometry. Applications include studying endothelial and lymphatic development and FOXC2-ETS cooperation. Particles are purified by PEG precipitation and sucrose gradient centrifugation for transduction of primary and thawed cells.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FOXC2 & ETS family members synergistic activity transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
