| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
FOXD1 is a forkhead box transcription factor with a conserved winged-helix DNA-binding domain that regulates genes essential for developmental patterning and tissue maintenance. It has well-defined roles in kidney and retina development, where it directs stromal cell behavior, as well as in lymphatic vessel formation and embryo implantation. Among its target genes is placental growth factor, a VEGF family member involved in angiogenesis. FOXD1 has been associated with several human conditions, including cancer and recurrent pregnancy loss, where altered FOXD1 activity affects tissue development and homeostasis. These functions make FOXD1-dependent transcription an informative readout in developmental and disease biology research.
Product Description & Applications
The FOXD1 Reporter Lentivirus is a transcription-factor reporter system designed to preferentially detect FOXD1 transcriptional activity in human and mouse cells. The construct uses tandem repeats of the FOXD1 DNA-binding element derived from the human placental growth factor promoter, a validated downstream target, to drive a fluorescent or luminescent reporter, with options including GFP, RFP, mCherry, BFP2, and luciferase configurations. A constitutively expressed selection marker supports stable polyclonal cell line generation.
Readout is by microscopy, flow cytometry, or luminometry. Applications include studying FOXD1 in kidney and retina development, lymphatic biology, and related disease processes. Particles are purified by PEG precipitation and sucrose gradient centrifugation for transduction of primary and thawed cells.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FOXD1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
