FoxD4/D4L Polyclonal Antibody

SKU:BHA12104724
Overview
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FOXD4-specific polyclonal antibody raised in Rabbit. Validated for Western Blot, ELISA in Human samples. Affinity-purified rabbit IgG.
Specificity Total Protein
Immunogen Synthetic Peptide
Molecular Weight ~45.8 kDa
UniProt Q12950
Gene ID Human: 653404/2298/100036519/349334/6534
Applications 2 validated
Options selector
Catalog no. Size
BT-AP03312-20UL 20 uL
BT-AP03312-50UL 50 uL
BT-AP03312-100UL 100 uL
Available Options
Size
Field Specification
Mfr No BT-AP03312
Alternative Names FOXD4; FKHL9; FOXD4A; FREAC5; Forkhead box protein D4; Forkhead-related protein FKHL9; Forkhead-related transcription factor 5; FREAC-5; Myeloid factor-alpha; FOXD4L4; FOXD4B; Forkhead box protein D4-
Clonality
  • Polyclonal
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID Human: 653404/2298/100036519/349334/653427
Host Rabbit
Immunogen The antiserum was produced against synthesized peptide derived from human FOXD4/L2/L3/L4/L5/L6. AA range:281-330
Isotype
  • IgG
Molecular Weight ~45.8 kDa
Product Type
  • Primary Antibody
Reactivity
  • Human
Storage -20°C for 1 year
Target FOXD4

Product Overview

Primary Antibody

This antibody targets FOXD4 and detects endogenous levels of FOXD4 protein.

Validated Applications

Application Dilution Guidance
Western Blot (WB) Refer to datasheet for recommended working dilution
ELISA Refer to datasheet for recommended working dilution
✔ Validated by Bioassay Technology Laboratory

Antibody Specifications

Target FOXD4
Host Rabbit
Clonality Polyclonal
Isotype IgG
Reactivity Human
Purification Affinity Purification
Molecular Weight ~45.8 kDa
Concentration 1 mg/ml
Immunogen The antiserum was produced against synthesized peptide derived from human FOXD4/L2/L3/L4/L5/L6. AA range:281-330
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -20°C for 1 year
Gene ID Human: 653404/2298/100036519/349334/653427
UniProt Q12950 (Human: Q12950/Q8WXT5/Q5VV16/Q6VB85/Q6VB84/Q3SYB3)

Scientific Background

Forkhead box protein D4 (FOXD4)also named as FKHL9 or Myeloid factor alpha is a 439 amino acid protein, which contains one fork head DNA-binding domain. FOXD4L6 localizes in the nucleus. FOXD4 as an embryonic transcriptional regulator involves in leukemogenesis. The molecular weight of FOXD4L6 is 47kDa, but our experiment always detected a 60-70kDa protein by western blot.

Alternative Names

FOXD4; FKHL9; FOXD4A; FREAC5; Forkhead box protein D4; Forkhead-related protein FKHL9; Forkhead-related transcription factor 5; FREAC-5; Myeloid factor-alpha; FOXD4L4; FOXD4B; Forkhead box protein D4-

Safety & Handling

⚠ Research Use Only (RUO). Not approved for diagnostic or therapeutic use. Handle with standard laboratory safety precautions. Contains sodium azide — avoid contact with skin/eyes; dispose per local regulations.
TargetFOXD4
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ReactivityHuman
PurificationAffinity Purification
Molecular Weight~45.8 kDa
Concentration1 mg/ml
ImmunogenThe antiserum was produced against synthesized peptide derived from human FOXD4/L2/L3/L4/L5/L6. AA range:281-330
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage-20°C for 1 year
Gene IDHuman: 653404/2298/100036519/349334/653427
UniProtQ12950 (Human: Q12950/Q8WXT5/Q5VV16/Q6VB85/Q6VB84/Q3SYB3)

Optimized working dilutions depend on sample type and detection system. Titration is recommended.

ApplicationDilution Note
Western Blot (WB)Refer to datasheet for recommended working dilution
ELISARefer to datasheet for recommended working dilution

Forkhead box protein D4 (FOXD4)also named as FKHL9 or Myeloid factor alpha is a 439 amino acid protein, which contains one fork head DNA-binding domain. FOXD4L6 localizes in the nucleus. FOXD4 as an embryonic transcriptional regulator involves in leukemogenesis. The molecular weight of FOXD4L6 is 47kDa, but our experiment always detected a 60-70kDa protein by western blot.

Alternative Names

FOXD4; FKHL9; FOXD4A; FREAC5; Forkhead box protein D4; Forkhead-related protein FKHL9; Forkhead-related transcription factor 5; FREAC-5; Myeloid factor-alpha; FOXD4L4; FOXD4B; Forkhead box protein D4-

What species does this antibody react with?
This antibody has been validated for reactivity against Human. Actual cross-reactivity is determined by immunogen sequence homology across species. Contact technical support for unlisted species.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB), ELISA. Each application was tested with positive and negative controls. Contact our team regarding applications not listed.
How was this antibody produced and purified?
This polyclonal IgG antibody was raised in rabbit against a synthesized target-specific peptide. It was affinity-purified from antiserum using an epitope-specific immunogen, yielding a highly specific antibody suitable for multiple research applications.
What is the expected molecular weight of FOXD4?
The predicted molecular weight of FOXD4 is ~45.8 kDa. Band size may vary slightly depending on post-translational modifications and gel percentage.
What storage conditions does this antibody require?
Store at −20°C for long-term storage. Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for short-term use. The formulation includes 50% glycerol as a cryoprotectant.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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