| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
FOXK1 is a member of the forkhead box family of transcription factors, characterized by a conserved winged-helix DNA-binding domain. It functions as either a transcriptional activator or repressor depending on cellular context, and regulates glucose and lipid metabolism, aerobic glycolysis, muscle cell differentiation, and autophagy. FOXK1 and the closely related FOXK2 show partial functional overlap but distinct regulatory roles across physiological and disease states. In response to insulin, FOXK1 translocates from the cytoplasm to the nucleus in an Akt-mTOR-dependent manner to direct metabolic reprogramming. These activities place FOXK1 at the intersection of nutrient signaling, cell differentiation, and metabolic disease research.
Product Description & Applications
The FOXK1 Reporter Lentivirus is a transcription-factor reporter system that detects FOXK1-mediated transcription in human and mouse cells. The construct uses tandem repeats of consensus FOXK1 DNA-binding elements coupled to a minimal promoter to drive a fluorescent or luminescent reporter, with options including GFP, RFP, mCherry, BFP2, firefly luciferase, Gaussia luciferase, Renilla luciferase, and dual configurations. A constitutively expressed selection marker supports stable polyclonal cell line generation, with readout by microscopy, flow cytometry, or luminometry.
Applications include studying FOXK1 in glucose and lipid metabolism, autophagy, muscle differentiation, and insulin-Akt-mTOR signaling. Particles are purified by PEG precipitation and sucrose gradient centrifugation for effective transduction of primary and thawed cells.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FOXK1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
