| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
FOXO3 is a member of the FOXO subfamily of Forkhead box transcription factors that act as key effectors of the PI3K/AKT signaling pathway. When AKT is inactive, FOXO3 enters the nucleus and binds Forkhead response elements to activate genes controlling cell cycle arrest, apoptosis, DNA repair, oxidative stress resistance, and metabolism. FOXO3 contributes to stem cell maintenance, longevity, and tumor suppression, and its activity is opposed by growth factor signaling. FOXO3 is widely studied in aging, cell survival, and cancer research, and shares features with the related factor FOXO1.
Product Description & Applications
The FOXO3 Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem consensus response elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of FOXO3 activity. The reporter is preferentially activated by FOXO3 rather than FOXO1 and can also read out transcriptional inhibition by FOXP1. Reporter options include EGFP, GFP, mCherry, RFP, firefly luciferase, Renilla luciferase, and a β-Lactamase-V5 reporter, with optional blasticidin or puromycin selection. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.
It supports stable reporter cell line generation for studying FOXO3 signaling in cell survival and stress responses.
About This Product
This reporter lentivirus places a EGFP, Firefly Luc, GFP, Luc, mCherry, Renilla Luc, RFP, β-Lactamase-V5 reporter gene under the control of tandem consensus response elements specific for the FOXO3 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
