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The FRTL-5 cell line, derived from normal rat thyroid follicular cells, plays a significant role in thyroid research, particularly focusing on the physiology and pathophysiology of the gland. These cells are characterized by their dependence on thyroid-stimulating hormone (TSH) for proliferation, making them an essential model for studying TSH regulation and thyroid hormone biosynthesis. Importantly, FRTL-5 cells retain the capability to uptake iodide, which is crucial for investigating iodide metabolism and the production of thyroid hormones. This feature underscores their utility in exploring thyroid function and dysfunctions.
In addition to their fundamental roles in thyroid hormone studies, FRTL-5 cells have been instrumental in examining the influence of growth factors, cytokines, and oncogenes on thyroid biology. Their consistent expression of thyroid-specific markers, including thyroglobulin and thyroperoxidase, makes them valuable for molecular and cellular biology studies aimed at understanding thyroid-related diseases. As such, FRTL-5 cells are frequently utilized in research addressing thyroid cancer, autoimmune thyroid disease, and other related disorders, contributing significant insights into the cellular mechanisms driving these conditions.
Moreover, the FRTL-5 cell line has been critical in research related to autoimmune thyroid disorders such as Graves' disease. It has been used to assay the activity of immunoglobulins in human samples, offering a robust and reproducible model for studying autoimmune interactions with thyroid cells. The three-dimensional growth pattern of these cells provides a more physiologically relevant environment for examining cell behavior and intercellular interactions in thyroid biology. These attributes, combined with decades of research leveraging FRTL-5 cells, underscore their importance in advancing our understanding of thyroid health and disease.
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- cultureMedium: Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a)
- supplements: Supplement the medium with 5% FBS, 10 mg/L Insulin, 5 mg/L Transferrin, 50 microgram/L Hydrocortison, 10 microgram/L Somatostatin, 10 microgram/L Gly-His-Lsy-acetate, 0.0165 microgram/mL bovine TSH (catalog number T1614 from Scripps Laboratories) - Add the required TSH just before use and sterile filter into the medium.
- dissociationReagent: Accutase
- doublingTime: 30-34 hours
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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