| Field | Specification |
|---|---|
| Mfr No | |
| Species |
FRTL (Fischer Rat Thyroid Low Serum) cells are a continuous line of rat thyroid follicular cells that have been cultured to study various aspects of thyroid physiology and pathology. These cells are particularly notable for their ability to accumulate iodide intracellularly, a key characteristic reflective of thyroid function in vivo. This unique feature makes them suitable for research focused on thyroid hormone biosynthesis, the mechanism of iodide transport, and the effects of various substances on thyroid function.
The culture conditions for FRTL cells are quite specific, requiring a specialized medium to maintain their physiological properties. Supplements such as FBS, insulin, hydrocortisone, thyrotropin, transferrin, somatostatin, and glycyl-1-histidyl-lysine acetate are necessary to replicate the hormonal environment of the thyroid gland. This precise combination of conditions supports the cells' typical growth pattern, where they tend to stack upon one another and form three-dimensional structures rather than spreading as a monolayer. This clustering behavior is significant as it mimics the follicular arrangement found in natural thyroid tissue, thus providing a more accurate model for studying thyroid cell interactions and dynamics in a controlled setting.
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- Tumorigenic: No
- Products: Thyroglobulin
- Karyotype: Diploid
- cultureMedium: Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a)
- supplements: Supplement the medium with 0.5% FBS, 10 mg/L Insulin, 5 mg/L Transferrin, 50 microgram/L Hydrocortison, 10 microgram/L Somatostatin, 10 microgram/L Gly-His-Lsy-acetate, 0.0165 microgram/mL bovine TSH (catalog number T1614 from Scripps Laboratories) - Add the required TSH just before use and sterile filter into the medium.
- dissociationReagent: Accutase
- doublingTime: 5-7 days
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- fluidRenewal: 3 times per week
- postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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