| Field | Specification |
|---|---|
| Mfr No | |
| Species |
The FS-Balb cell line is a murine fibroblast cell line derived from the skin of Balb/c mice. This cell line is used extensively in the field of dermatological research due to its origin and characteristics that mimic those of primary fibroblasts. The cells exhibit a fibroblastic morphology and are utilized in studies focused on skin biology, wound healing, and fibrosis. The robust proliferation rate of FS-Balb cells makes them a valuable model for in vitro experiments that require a consistent supply of fibroblast cells.
Genetically, FS-Balb cells retain many of the characteristics of Balb/c-derived fibroblasts, including their response to cytokines and growth factors. They are particularly useful for studying the interactions between skin cells and the immune system, which is critical in understanding inflammatory skin conditions. Moreover, these cells are often employed in genetic manipulation studies to explore gene function and regulation in a controlled environment. The compatibility of FS-Balb cells with various transfection methods supports their use in overexpression and knockdown experiments, which are essential for dissecting cellular pathways and mechanisms relevant to skin health and disease.
—
- cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 1 to 2 x 104 cells/cm2
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
10%
OFF
15%OFF
20%
OFF
$50
OFF
FREE
SAMPLE
