FS-C57BL cell

SKU:BHC11100510
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Overview
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FS-C57BL cell is a Fibroblast cell line (Female). It is commonly used as an in vitro model for 1 research. Growth characteristics: Adherent. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Mouse
Disease model Sarcoma
Growth Properties Adherent
Tissue Skin
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Catalog no. Size
400420 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 400420
Species Mouse
FS-C57BL is a fibrosarcoma cell line derived from C57BL mice, commonly utilized in cancer research. The origin of this cell line traces back to spontaneous tumor formations in these mice, which are genetically modified to be predisposed to cancer. The FS-C57BL cell line is significant for its robust growth and reproducibility in experimental settings, making it a valuable tool for studying cancer biology, especially in the context of fibrosarcoma. The cell line exhibits characteristics typical of sarcomas, including high mitotic index and the ability to form tumors when inoculated into compatible hosts. In research, FS-C57BL is often used to explore the cellular mechanisms underlying fibrosarcoma progression and metastasis. It serves as a model to assess the efficacy of chemotherapeutic agents and to study the genetic and molecular pathways involved in tumor growth and response to treatment. Researchers also leverage this cell line to investigate immune responses in cancer, benefiting from the C57BL mouse's well-documented immune profile. Thus, FS-C57BL aids in bridging in vitro experiments with in vivo outcomes, enhancing the translational relevance of the research conducted with these cells.

SKU:BHC11100510

  • cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
  • supplements: Supplement the medium with 10% FBS
  • dissociationReagent: Accutase
  • subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
  • seedingDensity: 1 x 104 cells/cm2 will yield in a confluent layer in about 2to3 days
  • fluidRenewal: 2 to 3 times per week
  • postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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