FTX-3.3

Product details

• Chemical name: 1, 4-Dihydro-2,6-dimethyl-4-(2-nitrophenyl)-3,5-pyridinedicarboxylic acid dimethyl ester.
• Also known as: Funnel Web Spider Toxin
• PubChem CID: 9970537
• Molecular formula: C12H31N7.
• Purity: >99%
• Concentration: 0.1-1 mM.
• Form: Lyophilized powder.
• Solubility: Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
• Reconstitution: Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
• Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
• Target: P-type Ca2+ channels
• Source: Synthetic
• Activity: FTX-3.3 is a synthetic polyamine1-2, reputed to be structurally identical to the native Funnel Web Spider Toxin (FTX). It blocks P-, N-, and L-type Ca2+ channels in mammalian Purkinje and superior cervical ganglia neurons with similar potencies3.
• Alternative names: Funnel Web Spider Toxin

Scientific background

FTX is a polyamine component of the venom of the American funnel web spider Agelenopsis aperta, and has been described as a selective antagonist of P-type voltage-dependent Ca2+ channels1-2. However, it has been demonstrated that FTX has actions at other sites including NMDA and GABA receptors and also on T-type calcium channels3.FTX-3.3 (reputed to be structurally identical to the native FTX) has been synthesized4-5 and shown to block P-type calcium channels in rat cerebellar Purkinje cells6. It antagonizes P-, N-, and L-type calcium channels in mammalian Purkinje and superior cervical ganglia neurons with similar potencies; Its IC50 against P-, N-, and L-type channels are ~0.13 mM, ~0.24 mM and ~0.24 mM, respectively7.FTX-3.3 does not reduce whole-cell sodium current recorded from SK.N.SH cells and is therefore ion selective8.

Lead time: 1-2 Business Days

Country of origin: Israel/IL

Applications key

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Bioassay tested: Yes

• SDS (PDF)

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