| Field | Specification |
|---|---|
| Mfr No | |
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
Fully characterized Human Preadipocytes Subcutaneous, Diabetic Adult (HPA-CD) are primary human preadipocytes isolated from the subcutaneous adipose tissue of a diabetic adult donor. These cells are extensively characterized for phenotype, growth, and differentiation potential, providing a physiologically relevant model for studying adipogenesis, metabolic disease, insulin resistance, and diabetes-related research. The product is supplied as frozen cells.
Key elements and design rationale
- Biological source: Human (H. sapiens)
- Tissue origin: Adipose
- Growth properties: Adherent, fibroblast-like
- Format: Frozen
- Donor history: Female, 58, Caucasian, BMI 26.7, Type 2 Diabetes, Medication (Glucophage, Pariet, Simvastatin, and Coveram)
- Biosafety level: II
- Culture context: Cell Expansion and Maintenance:PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture.
Adipose-lineage cells are used to study differentiation, lipid handling, endocrine signaling, and inflammatory remodeling in adipose tissue models.
Research relevance and current trends
- Primary adipose-lineage cells are used to compare donor-specific adipogenic capacity and metabolic responsiveness.
- Researchers frequently assess inflammatory remodeling and adipokine-associated signaling in differentiated cultures.
- Differentiation-state tracking remains central to adipose tissue modeling and screening workflows.
Common research applications
- Monitor adipogenic differentiation and lipid-associated marker changes over time.
- Study metabolic or inflammatory responses in adipose-relevant culture systems.
- Use in conditioned-media or co-culture assays to probe adipose crosstalk.
Product-specific data supplied for this listing
- Growth Conditions: Cell Expansion and Maintenance:PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Preadipocyte Medium Kit (TM048) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Adipocyte Differentiation:PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture.
- Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078) or, if serum is preferred, Cryopreservation Medium (TM024).
- Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
