| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping | |
| Species |
Background
FXRα (Farnesoid X Receptor alpha, NR1H4) is a bile acid-activated nuclear receptor that functions as a master regulator of bile acid, lipid, and glucose metabolism. Activated by bile acids, FXRα heterodimerizes with the retinoid X receptor and binds FXR response elements to control genes involved in bile acid synthesis, transport, and enterohepatic circulation. It is highly expressed in the liver and intestine, where it provides feedback regulation that protects cells from bile acid toxicity and coordinates metabolic homeostasis. Because of its central roles, FXRα is an important drug target in cholestatic liver disease, nonalcoholic fatty liver disease, and metabolic disorders.
Product Description & Applications
This ORF cDNA lentivirus enables stable overexpression of human FXR (Farnesoid X Receptor) in mammalian cells. A CMV or EF1α promoter drives the FXR ORF fused to a C-terminal V5 epitope tag, followed by a GFP or RFP reporter separated by a self-cleaving peptide for independent translation from a single transcript; in some configurations the reporter and a puromycin or blasticidin selection marker are driven by a PGK promoter. ORF accuracy has been confirmed by sequencing and expression validated by transient transfection.
Delivered as a third-generation, VSV-G-pseudotyped system, the PEG- and sucrose-gradient-purified particles transduce a wide range of cells, including primary and thawed cultures, and support reliable genome integration for long-term stable lines. The product supports research on FXR in bile acid and lipid metabolism and FXR-targeted drug development.
About This Product
This ORF cDNA lentivirus enables stable overexpression of FXR (NCBI Accession: NM_001206979.2, NM_005123) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.
Ultra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
