| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
The farnesoid X receptor response element (FXRE) is a DNA motif bound by the farnesoid X receptor (FXR, encoded by NR1H4), a ligand-activated nuclear receptor that serves as the principal sensor of bile acids. Activated by bile acids, FXR heterodimerizes with the retinoid X receptor and binds FXREs to regulate genes controlling bile acid synthesis, transport, and enterohepatic circulation, as well as lipid and glucose metabolism. FXR also exerts protective and anti-inflammatory effects in the liver and intestine. Because of its central role in metabolic homeostasis, FXR signaling is an important target in research on cholestasis, fatty liver disease, and metabolic disorders.
Product Description & Applications
The FXRE Reporter Lentivirus places a reporter gene under the control of tandem farnesoid X receptor response elements, providing a sensitive readout of FXR signaling pathway activity in human and mouse cells. Reporter options include fluorescent (GFP, RFP) and luminescent (firefly and Renilla luciferase) formats, with puromycin or blasticidin selection for stable reporter cell line establishment. Signal can be measured by fluorescence microscopy, flow cytometry, or luminometry.
The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring FXR activation, studying bile acid and lipid metabolism, and screening FXR agonists and antagonists.
About This Product
This reporter lentivirus places a Firefly Luc, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the Farnesoid X receptor pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
