| Field | Specification |
|---|---|
| Applications | |
| Sterility | Negative for bacteria, yeast, fungi, and mycoplasma. |
| Cell type | |
| Form | Liquid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
GABAergic neurons are a type of neuron that produce gamma-aminobutyric acid (GABA), a neurotransmitter that inhibits nerve transmission and reduces neuronal excitability mainly in hippocampus, thalamus, basal ganglia, hypothalamus, and brainstem [1]. The balance between inhibitory neuronal transmission via GABA and excitatory neuronal transmission via glutamate is essential for proper cell membrane stability and neurologic function in central nervous system (CNS). In GABAergic neurons, GABA is primarily synthesized from glutamate, a process catalyzed by glutamate decarboxylase (GAD). GAD, especially the CNS specific 67 kDa subtype GAD67, as well as vesicular GABA neurotransmitter transporter (vGAT) are commonly used cell specific markers for GABAergic neurons [2].
GABAergic Neuron Culture Medium is a sterile-filtered, ready-to-use liquid medium from iXCells Biotechnologies for the routine culture of GABAergic Neurons in vitro. Each lot is screened for bacteria, yeast, fungi and mycoplasma before release.
Scientific Background
iXCells® GABAergic Neuron Culture Medium is a complete medium uniquely designed to maintain GABAergic neuron growth in vitro and their functions, as measured by the expression of the cell type specific markers along with the electrophysiology activities. The medium is provided as the antibiotics free complete culture medium. No extra glutamine is needed for cell culture.
Specifications
| Catalog No. | MD-0122 |
|---|---|
| Package Size | 100 mL |
| Form | Liquid |
| Sterility | Negative for bacteria, yeast, fungi, and mycoplasma. |
| Storage | Store at -20°C. Protect from light. |
| Shipping | Dry ice |
| Country of Manufacture | United States of America (USA) |
Note: Please store the medium immediately at -20°C upon arrival.
Product Details
- Mammalian cells
- iPS cells and derivatives
- Serum free
- With phenol red
- Antibiotics free
QC & Validation
Lot-release testing
Tested in the iXCells Human Cortical GABAergic Neurons 2.0 (Cat. 40HU-022) following the recommended protocol.
Usage Notes
Do not use components that are beyond the expiration date indicated on the label. Tested in the iXCells Human Cortical GABAergic Neurons 2.0 (Cat: 40HU-022) following the recommended protocol in a 37°C, 5% CO2 incubator. Not for use in diagnostic procedures. Results may vary due to variations among tissue origin or donors. Results may vary due to different culture conditions including temperature, CO2 concentration, culture vessels, coating conditions, medium adding amount, and medium change frequency. For certain culture or test conditions, please follow the culture protocol for each specific cell type. iXCells in-house cell culture test was performed under antibiotics-free condition for ≥3 days by following the recommended protocol, and no bacterial or fungi contamination were observed during the culture period. Optional: Antibiotics-Antimycotic (Fisher, Cat# 15240062) or Penicillin Streptomycin (Thermo Fisher, Cat# 15140122) at working solution could be added if necessary, which have been tested in-house without visible effect on cell morphology or function.
Safety & Handling: When handling biohazardous materials such as cryopreserved cells, safe laboratory procedures should be followed, and personal protective equipment should be worn including but not limited to face masks, gloves, and lab coat.
Storage & Handling
For laboratory research use only. It is not approved for human or animal use.
Customization & Add-ons: Need this medium in a format that fits your protocol—or a formulation we don’t list? We can help you source the best match or support custom culture media for diverse research needs, including basal medium and complete medium formats, serum options (defined FBS lot, low-serum, serum-free or xeno-free), antibiotic preferences (antibiotic-free, Penicillin-Streptomycin or Antibiotic-Antimycotic), phenol red–free formulations, supplement and growth-factor adjustments (concentration changes, separate supplement packs, custom component kits), fill volume and packaging (small aliquots through bulk volumes), and lot reservation and repeat-order continuity for longitudinal studies (project dependent). We can also help you pair a medium with matched primary cells, coating reagents and dissociation solutions. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
