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| Selection Marker | Puromycin, Blasticidin, N/A |
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Background
The androgen receptor (AR), encoded by the NR3C4 gene, is a steroid hormone nuclear receptor that mediates the physiological actions of androgens such as testosterone and dihydrotestosterone. It is essential for the development and maintenance of reproductive tissues, muscle, and bone, and contributes to various metabolic processes. AR is highly expressed in androgen-responsive tissues, where it regulates genes that drive male characteristics and muscle growth. Upon binding hormone ligand, AR dissociates from accessory chaperones, translocates to the nucleus, and activates transcription of androgen-responsive genes. Because of its roles in endocrine physiology and cancer, AR is a major focus in oncology and endocrinology research.
Product Description & Applications
GAL4-DBD-AR-LBD-BSD is a GAL4-nuclear receptor reporter lentivirus that expresses a chimeric protein joining the yeast GAL4 DNA-binding domain to the ligand-binding domain (LBD) of the human androgen receptor. Upon ligand binding, the LBD recruits co-activators and the chimera binds upstream GAL4 response elements to drive a fluorescent or luminescent reporter (GFP, RFP, or firefly luciferase), with high signal-to-noise relative to endogenous nuclear receptor background.
The GAL4-LBD design isolates LBD-mediated transactivation and prevents cross-reactivity with endogenous receptor targets, enabling specific pharmacological characterization of AR agonists, antagonists, and selective androgen receptor modulators. Supplied as high-titer lentiviral particles, it allows generation of stable cell lines for ligand profiling and is amenable to high-throughput screening by luminometry.
About This Product
This lentiviral system expresses a chimeric protein consisting of the GAL4 DNA-binding domain fused to the ligand-binding domain (LBD) of AR. Upon specific ligand binding, the LBD undergoes conformational change and recruits co-activators; the activated chimera binds upstream GAL4 response elements (UAS×5) to drive expression of a fluorescent or luminescent reporter (GFP, RFP, or Firefly Luciferase) with high signal-to-noise relative to endogenous nuclear receptor background.
The GAL4-LBD design isolates LBD-mediated transactivation from A/B domain-driven constitutive activity and prevents cross-reactivity with endogenous nuclear receptor targets, enabling highly specific pharmacological characterization of LBD agonists, antagonists, and selective receptor modulators (SARMs/SERMs/SPPARMs). This system is established for drug discovery in academic and industrial settings, and is amenable to high-throughput screening (HTS) formats using luminometry.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
