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| Promoter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
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Background
PPARδ (PPARbeta/delta), encoded by PPARD and also designated NR1C2, is a ligand-activated transcription factor of the nuclear receptor superfamily. Together with PPARα and PPARγ, it coordinates lipid and energy metabolism, with each subtype playing distinct roles in metabolic regulation. PPARδ is broadly expressed, with high levels in skeletal muscle, liver, and heart, where it promotes fatty acid oxidation and energy expenditure and contributes to muscle physiology and inflammation control. Upon ligand binding, PPARδ heterodimerizes with RXR and regulates target genes through its ligand-binding domain. Its central role in energy homeostasis makes PPARδ an important target in research on metabolic disease and exercise physiology.
Product Description & Applications
GAL4-DBD-PPARδ-LBD-BSD is a GAL4-nuclear receptor reporter lentivirus that expresses a chimeric protein joining the yeast GAL4 DNA-binding domain to the ligand-binding domain (LBD) of human PPARδ. Upon ligand binding, the LBD recruits co-activators and the chimera binds upstream GAL4 response elements to drive a fluorescent or luminescent reporter (GFP, RFP, or firefly luciferase), with high signal-to-noise relative to endogenous nuclear receptor background.
The GAL4-LBD design isolates LBD-mediated transactivation and avoids cross-reactivity with endogenous receptor targets, enabling specific pharmacological characterization of PPARδ agonists, antagonists, and selective modulators. Supplied as high-titer lentiviral particles, it allows generation of stable cell lines for ligand profiling and is amenable to high-throughput screening by luminometry.
About This Product
This lentiviral system expresses a chimeric protein consisting of the GAL4 DNA-binding domain fused to the ligand-binding domain (LBD) of PPARδ. Upon specific ligand binding, the LBD undergoes conformational change and recruits co-activators; the activated chimera binds upstream GAL4 response elements (UAS×5) to drive expression of a fluorescent or luminescent reporter (GFP, RFP, or Firefly Luciferase) with high signal-to-noise relative to endogenous nuclear receptor background.
The GAL4-LBD design isolates LBD-mediated transactivation from A/B domain-driven constitutive activity and prevents cross-reactivity with endogenous nuclear receptor targets, enabling highly specific pharmacological characterization of LBD agonists, antagonists, and selective receptor modulators (SARMs/SERMs/SPPARMs). This system is established for drug discovery in academic and industrial settings, and is amenable to high-throughput screening (HTS) formats using luminometry.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
