| Field | Specification |
|---|---|
| Target | |
| Alternative names | Histone H2AX (H2a/x)|Histone H2A.X|H2AX|H2AFX |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic phosphorylated peptide around S139 of human H2AX |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes γ-H2AX. It is validated for Western Blot, Immunohistochemistry, Immunofluorescence.
Validated Applications
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence |
|---|---|
| WB | 1:500-1:1000 |
| IHC | 1:20-1:200 |
| IF | 1:20-1:100 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
A synthetic phosphorylated peptide around S139 of human H2AX
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | A synthetic phosphorylated peptide around S139 of human H2AX |
| Purification | Immunogen Affinity Purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Target MW | 17 kDa |
| UniProt # | P16104 |
| Gene ID | 3014 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
| Alternative Names | Histone H2AX (H2a/x)|Histone H2A.X|H2AX|H2AFX |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for γ-H2AX research from Fine Test, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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