| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
GAS (gamma-interferon-activated site) elements are DNA-binding sites that mediate the transcriptional response to interferon-gamma through the JAK/STAT signaling pathway. Interferon-gamma binding to its receptor activates the receptor-associated Janus kinases JAK1 and JAK2, which phosphorylate STAT1. Phosphorylated STAT1 forms homodimers that translocate to the nucleus and bind GAS elements in the promoters of interferon-stimulated genes, driving programs involved in antiviral defense, antigen presentation, macrophage activation, and immune regulation. This IFN-gamma/JAK/STAT1 axis is central to innate and adaptive immunity, and its dysregulation contributes to autoimmune and inflammatory disease as well as to tumor immune responses.
Product Description & Applications
The GAS Reporter Lentivirus is a lentiviral reporter system that provides a sensitive fluorescent or luminescent readout in response to gamma-interferon-activated site (GAS) transcriptional activation in human and mouse cells. GAS response elements drive reporter expression upon STAT1 activation, giving a quantitative measure of IFN-gamma/JAK/STAT1 pathway activity. The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cultures. A constitutively expressed selection marker (puromycin or blasticidin) supports stable reporter cell line generation, and the choice of fluorescent, luminescent, and SEAP reporters supports readout by fluorescence microscopy, flow cytometry, or luminometry for studies of interferon signaling, immune regulation, and pathway-modulating compounds.
About This Product
This reporter lentivirus places a Firefly Luc, Renilla Luc, SEAP, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the IFNg/JAK/STAT1 pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
