| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
GATA1 (GATA binding protein 1) is a zinc-finger transcription factor that recognizes GATA DNA motifs in the regulatory regions of its target genes. It is a master regulator of hematopoiesis, essential for the development and maturation of erythroid cells, megakaryocytes, eosinophils, and mast cells. GATA1 activates lineage-specific genes such as the globins while repressing programs of alternative fates, often acting together with cofactors including FOG1. Mutations and dysregulation of GATA1 are linked to inherited anemias and thrombocytopenias and to hematologic malignancies, including leukemias associated with Down syndrome. Reporter systems based on GATA-binding elements provide a quantitative readout of GATA1 transcriptional activity.
Product Description & Applications
The GATA1 Reporter Lentivirus is a transcription-factor reporter system for detecting GATA1-dependent transcription in mammalian cells. The construct uses tandem repeats of GATA1 DNA-binding elements derived from the human β-globin promoter, coupled to a minimal promoter that drives a fluorescent or luminescent reporter. A constitutively expressed selection marker and optional secondary reporter allow generation of stable polyclonal reporter cell lines with readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable lentiviral integration provides consistent, representative reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding the variability of transient transfection. Particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, supporting research on GATA1 in erythroid and megakaryocytic development and hematologic disease.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the GATA1 Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
