| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
GATA3 is a zinc-finger transcription factor of the GATA family that recognizes GATA DNA-binding motifs to control gene expression in development and immunity. It is the master regulator of T-helper 2 (Th2) cell differentiation and is essential for T lymphoid development and immune regulation. GATA3 is also expressed in multipotent hematopoietic stem cells, where it helps balance self-renewal and differentiation, and it has key roles in mammary gland and skin development. GATA family members GATA1, GATA2, and GATA3 share overlapping but distinct binding preferences; GATA1 and GATA2 are central to erythroid and megakaryocytic lineage development and early hematopoietic cell survival. Altered GATA3 function is implicated in immune disorders and breast cancer.
Product Description & Applications
The GATA3 Reporter Lentivirus is a lentiviral transcription-factor reporter system that provides a sensitive fluorescent or luminescent readout of GATA3 transcriptional activity in mammalian cells. The construct contains tandem repeats of consensus GATA3 DNA-binding elements that drive reporter expression in response to GATA3, coupled to a minimal promoter for high signal-to-noise. The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cultures. A constitutively expressed selection marker (puromycin or blasticidin) supports stable reporter cell line generation, and the choice of reporters supports readout by fluorescence microscopy, flow cytometry, or luminometry for studies of Th2 differentiation, hematopoiesis, immune regulation, and GATA3-targeted compounds.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the GATA3 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
