| Field | Specification |
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| Assay type | |
| Assay target | |
| Assay area | |
| Target type | |
| Detection method | |
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Drug Target Assay Kit
Human topoisomerase II alpha decatenates (unlinks) concatenated DNA. This gel-based kit provides the enzyme, concatenated DNA and reagents for 100 decatenation reactions.
Kit Components
| Component | HDD100KEG |
|---|---|
| 10 x Assay buffer | 300 µl |
| 10 x Concatenated DNA (30 µg/ml) | 200 µl |
| 10 x ATP | 200 µl |
| 0.5 M EDTA | 200 µl |
| 200 x human topoisomerase II alpha (10 U/µl) | 15 µl |
| Enzyme dilution buffer | 1 ml |
| 5 x Gel-loading buffer | 500 µl |
Specifications
| Assay Target | Human topo II DNA decatenation |
|---|---|
| Target Type | Topoisomerase |
| Organism | Human |
| Assay Type | Enzyme Activity Assay |
| Detection Method | Gel Electrophoresis |
| Size | 100 assays |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped frozen. |
Protocol
A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Safety & Handling
For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The Gel-Based Human Topoisomerase II DNA Decatenation Assay Kit Plus is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.
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Q.What does this kit measure?
A.It measures the DNA decatenation activity of human topoisomerase II alpha on concatenated DNA, with the reaction products analyzed by gel electrophoresis.
Q.What is included in the kit?
A.The Gel-Based Human Topoisomerase II DNA Decatenation Assay Kit Plus (100 assays) includes 10 x assay buffer (300 µl), 10 x concatenated DNA (30 µg/ml) (200 µl), 10 x ATP (200 µl), 0.5 M EDTA (200 µl), 200 x human topoisomerase II alpha (10 U/µl) (15 µl), Enzyme dilution buffer (1 ml) and 5 x Gel-loading buffer (500 µl).
Q.Is a protocol available?
A.A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Q.How should the kit be stored?
A.The Gel-Based Human Topoisomerase II DNA Decatenation Assay Kit Plus is shipped frozen. Please contact us for long-term storage conditions.
Can’t find the exact format you need? We can help you source or customize this human topo II DNA decatenation assay — including larger pack sizes, bulk reagents, bulk enzyme, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.
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