Gel-Based SARS-CoV-2 RdRp Assay Kit

SKU:BHT16300022
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Overview
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Gel-based SARS-CoV-2 RdRp Assay Kit for testing the activity of the SARS-CoV-2 RNA-dependent RNA polymerase and its inhibitors. RNA products are read by gel electrophoresis using the supplied gel loading buffer. One 20-assay size with RNA template, NTPs and RdRp enzyme included.
Assay Target SARS-CoV-2 RdRp activity
Detection Method Gel Electrophoresis
Options selector
Catalog no. Size
GS2RPA020KE 20 assays
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 20 assays
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: Please contact us to confirm storage conditions.
  • Shipping: Shipped frozen.
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Organism SARS-CoV-2
Applications
  • Enzyme Activity
  • Compound Profiling
Assay type
  • Enzyme Activity Assay
  • Inhibitor Screening Assay
Assay target SARS-CoV-2 RdRp activity
Assay area Antiviral Drug Discovery
Target type RNA Polymerase
Detection method
  • Gel Electrophoresis
Storage Please contact us to confirm storage conditions.
Shipping Shipped frozen.
Catalog no. (Mfr.) GS2RPA020KE
Main SKU BHT16300022
Drug Target Assay Kit

SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) copies the viral RNA genome and is a target for antiviral drug discovery.

Kit Components

Component GS2RPA020KE
10 x Buffer 75 µl
50 x RNA template 10 µl
50 x NTPs (ATP, UTP, GTP, CTP) 12 µl
50 x SARS-CoV-2 RdRp 10 µl
5 x Gel loading buffer 130 µl

Specifications

Assay Target SARS-CoV-2 RdRp activity
Target Type RNA Polymerase
Organism SARS-CoV-2
Assay Type Enzyme Activity Assay; Inhibitor Screening Assay
Detection Method Gel Electrophoresis
Size 20 assays
Storage Please contact us to confirm storage conditions.
Shipping Shipped frozen.

Protocol

A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.

Safety & Handling

For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The Gel-Based SARS-CoV-2 RdRp Assay Kit is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.
Q.What does this kit measure?
A.It measures the RNA synthesis activity of SARS-CoV-2 RNA-dependent RNA polymerase (RdRp), with RNA products checked on a gel. It can be used to test compounds that block this antiviral target.
Q.What is included in the kit?
A.The Gel-Based SARS-CoV-2 RdRp Assay Kit (20 assays) includes 10 x buffer (75 µl), 50 x RNA template (10 µl), 50 x NTPs (ATP, UTP, GTP, CTP) (12 µl), 50 x SARS-CoV-2 RdRp (10 µl) and 5 x gel loading buffer (130 µl).
Q.Is a protocol available?
A.A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Q.How should the kit be stored?
A.The Gel-Based SARS-CoV-2 RdRp Assay Kit is shipped frozen. Please contact us for long-term storage conditions.

Can’t find the exact format you need? We can help you source or customize this SARS-CoV-2 RdRp activity assay — including larger pack sizes, bulk reagents, bulk enzyme, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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