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Product Overview
This kit can recover high-purity DNA fragments containing no salt or low salt concentration and no impurities such as protein and RNA from various concentrations of agarose gel. The 200 bp–10 kb DNA fragment has a recovery rate of 80% or more, and single-stranded, double-stranded DNA fragments and circular plasmid DNA can be recovered. Recovered DNA can be used directly in restriction digestion, ligation and sequencing reactions.
The excised gel slice is weighed and dissolved in Solution G at 3 µL per mg of gel (300 µL per 100 mg), or 6 µL per mg for gels above 2%, at 55°C for 5–10 min. The solution is loaded onto Adsorption column G, washed with 300 µL of Solution G and twice with 600 µL of Wash Buffer, dried for 2 min at room temperature and eluted with 15–50 µL of buffer, with 30 µL recommended. Recovered DNA has an OD260/OD280 of about 1.8–2.0.
Specifications
| Format | Spin column (Adsorption column G) |
|---|---|
| Sample type | Agarose gel of various concentrations |
| Sample input | Maximum 300 mg gel per tube |
| Fragment range | 200 bp–10 kb |
| Recovery | 80% or more |
| Nucleic acids recovered | Single-stranded and double-stranded DNA fragments and circular plasmid DNA |
| Purity | OD260/OD280 is about 1.8–2.0 |
| Elution volume | 15–50 µL, 30 µL recommended |
| Processing time | 10 min |
Kit Components
| Component | 50 T | 200 T |
|---|---|---|
| Solution G | 60 mL | 240 mL |
| Wash Buffer | 60 mL | 240 mL |
| Elution Buffer | 5 mL | 20 mL |
| Adsorption column G | 50 set | 200 set |
Applications
- Recovery of DNA fragments from agarose gel
- Recovery of circular plasmid DNA
- Enzyme digestion and ligation of recovered fragments
- Sequencing reactions
Key Features
- Fast: The entire operation process is fast and convenient, and the recovery work can be completed in ten minutes.
- Variety: Can recover single-stranded, double-stranded DNA fragments and circular plasmid DNA.
- High efficiency: The unique spin column and carefully prepared buffer can recover a large amount of high-purity target DNA.
Storage & Handling
All components are stored at room temperature for 1 year.
Usage Notes
- Wear gloves when weighing and handling gel slices.
- Ensure the gel is completely dissolved before loading the column.
- Check that the pH of Solution G is 7.5 or below.
- Add ethanol to the Wash Buffer before first use.
- Extend the column drying time when the room temperature is low.
- Do not use polyacrylamide gels.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
Connexin 43 overexpression induces lung cancer angiogenesis in vitro following phosphorylation at Ser279 in its C‑terminus. Oncology Letters, 2022. 10.3892/ol.2022.13413
Phenotypic and Molecular Identification of R. dentocariosa Bacteria from Samples Isolated from the Oral Cavity.. JOURNAL of UNIVERSITY of BABYLON for Pure and Applied Sciences, 2025. 10.29196/jubpas.v33i1.5643
