| Field | Specification |
|---|---|
| Organism | |
| Applications | |
| Assay type | |
| Assay target | |
| Assay area | |
| Target type | |
| Detection method | |
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| Shipping | |
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| Main SKU |
Single-strand DNA-binding protein (SSB) binds single-stranded DNA, or single-stranded regions of double-stranded DNA, during DNA replication, recombination and repair; for example, DNA helicase unwinding creates single-strand DNA. E. coli SSB forms a homotetramer of 19 kDa subunits.
Kit vs Kit Plus
The only difference is that GSSB100KE (Kit Plus) also includes 150 µl of 2 mg/ml E. coli SSB protein; GSSB100K contains the buffer, ssDNA and loading buffer only, for use with your own SSB protein.
| Kit (SSB protein not included), 100 assays (GSSB100K) | 100 assays |
|---|---|
| Kit Plus (SSB protein included), 100 assays (GSSB100KE) | 100 assays |
Kit Components
| Component | Kit (SSB protein not included), 100 assays (GSSB100K) | Kit Plus (SSB protein included), 100 assays (GSSB100KE) |
|---|---|---|
| 10 x Buffer | 150 µl | 150 µl |
| 10 x ssDNA (45-mer single-strand DNA) | 150 µl | 150 µl |
| 4 x Gel loading buffer | 100 µl | 100 µl |
| E. coli SSB protein (2 mg/ml) | Not included | 150 µl |
Assay Principle
SSB is mixed with a 45-mer single-strand DNA and run on a conventional acrylamide gel. SSB-bound DNA migrates more slowly than free DNA, so binding activity shows as a band shift.
Specifications
| Assay Target | SSB single-strand DNA binding |
|---|---|
| Target Type | Single-strand DNA-binding protein |
| Organism | E. coli |
| Assay Type | Binding Assay |
| Detection Method | Gel Electrophoresis |
| Size | 100 assays |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped frozen. |
Protocol
The manufacturer's PDF describes the assay and kit contents; please contact us for the full procedure.
- Gel Shift Single-strand DNA Binding Assay (PDF, see Documents): Covers catalog # GSSB100K, GSSB100KE.
Safety & Handling
Can’t find the exact format you need? We can help you source or customize this SSB single-strand DNA binding assay — including larger pack sizes, bulk reagents, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.
